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Curve of the Michaelis–Menten equation labelled in accordance with IUBMB recommendations. In biochemistry, Michaelis–Menten kinetics, named after Leonor Michaelis and Maud Menten, is the simplest case of enzyme kinetics, applied to enzyme-catalysed reactions involving the transformation of one substrate into one product.
Used of an antibody-based method to detect a specific protein in a sample: Molecular biology, Biochemistry: Intracellular recording: Used to measure the voltage across a cell membrane: Neuroscience, Electrophysiology: Microarray
A threshold tracking experiment consists of a 1-ms stimulus being applied to a nerve in regular intervals. [10] The action potential is recorded downstream from the triggering impulse. The stimulus is automatically decreased in steps of a set percentage until the response falls below the target (generation of an action potential).
One such example of a spiking neuron model may be a highly detailed mathematical model that includes spatial morphology. Another may be a conductance-based neuron model that views neurons as points and describes the membrane voltage dynamics as a function of trans-membrane currents.
Pulse-chase analysis of auxin signal transduction in an Arabidopsis thaliana wildtype and an axr2-1 mutant. Wild-type and axr2-1 seedlings were labeled with 35S-methionine, and AXR2/axr2-1 protein was immunoprecipitated either immediately after the labeling period (t = 0) or following a 15-minute chase with unlabeled methionine (t = 15).
The question is now how to extract the orthotropic Engineering constants from the frequencies measured with IET on the beams and Poisson plate. This problem can be solved by an inverse method (also called" Mixed numerical/experimental method" [11]) based on a finite element (FE) computer model of the Poisson plate. A FE model allows computing ...
The methods in this section are primarily computational although they typically require data generated by wet lab experiments. Protein–protein docking , the prediction of protein–protein interactions based only on the three-dimensional protein structures from X-ray diffraction of protein crystals might not be satisfactory.
Bio-layer interferometry platforms achieve high throughput by utilizing a "Dip and Read" format. [1] The biosensor tips themselves are transported directly to the desired sample and "dipped" into their respective compartment, eliminating the needs for micro-fluidics and the complications (clogging, purification) that come with it.