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Lugol's iodine is a liquid made up of two parts potassium iodide for every one part elemental iodine in water. [8] Lugol's iodine was first made in 1829 by the French physician Jean Lugol. [7] [8] It is on the World Health Organization's List of Essential Medicines. [9] [10] Lugol's iodine is available as a generic medication and over the ...
Lugol's solution or Lugol's iodine (IKI) is a brown solution that turns black in the presence of starches and can be used as a cell stain, making the cell nuclei more visible. Used with common vinegar (acetic acid), Lugol's solution is used to identify pre-cancerous and cancerous changes in cervical and vaginal tissues during "Pap smear" follow ...
The image on the right stains with Lugol's iodine, and identifies a squamous cell carcinoma, which does not pick up the stain. Chromoendoscopy is a medical procedure wherein dyes (often the same stains used in histology) are instilled into the gastrointestinal tract at the time of visualization with fibre-optic endoscopy.
They are stained pink or red by the counterstain, [3] commonly safranin or fuchsine. Lugol's iodine solution is always added after addition of crystal violet to form a stable complex with crystal violet that strengthen the bonds of the stain with the cell wall. [4] Gram staining is almost always the first step in the identification of a ...
Hemiamyloidity in mycology refers to a special case of cell wall amyloidity where the blue staining by iodine only occurs when the tissue was pretreated with potassium hydroxide solution (KOH) or other strong bases, whereas direct application of iodine causes a red reaction when using Lugol's solution, but no reaction when using Melzer's reagent.
The composition of Schiller's iodine is the same as Lugol's iodine, the latter being more concentrated. When Schiller's iodine is not available, Lugol's iodine can be used as an alternative. Schiller's test is not specific for cervical cancer, as areas of inflammation, ulceration and keratosis may also not take up the stain. [2]
The starch was then stained with Lugol's iodine. Gel zymography is often used for the detection and analysis of enzymes produced by microorganisms. [ 7 ] This has led to variations on the standard protocol e.g. mixed-substrate zymography.
Nevertheless, the iodide in tincture of iodine used as a water disinfectant does supply more than adequate nutritional iodine, perhaps 30 or more times the recommended daily allowance per liter or quart. Application of tincture or Lugol's to the skin also results in absorption and bioavailability of some moderate fraction of the iodine.