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  2. Relay lens - Wikipedia

    en.wikipedia.org/wiki/Relay_lens

    In optics, a relay lens is a lens or a group of lenses that receives the image from the objective lens and relays it to the eyepiece. Relay lenses are found in refracting telescopes , endoscopes , and periscopes to optically manipulate the light path , extend the length of the whole optical system , and usually serve the purpose of inverting ...

  3. Stereo microscope - Wikipedia

    en.wikipedia.org/wiki/Stereo_microscope

    The stereo, stereoscopic or dissecting microscope is an optical microscope variant designed for low magnification observation of a sample, typically using light reflected from the surface of an object rather than transmitted through it. The instrument uses two separate optical paths with two objectives and eyepieces to provide slightly ...

  4. Fluorescence microscope - Wikipedia

    en.wikipedia.org/wiki/Fluorescence_microscope

    A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption, to study the properties of organic or inorganic substances.

  5. Optical transfer function - Wikipedia

    en.wikipedia.org/wiki/Optical_transfer_function

    Although typically only a one-dimensional, or sometimes a two-dimensional section is used, the three-dimensional optical transfer function can improve the understanding of microscopes such as the structured illumination microscope. True to the definition of transfer function, () = should indicate the fraction of light that was detected from the ...

  6. Point spread function - Wikipedia

    en.wikipedia.org/wiki/Point_spread_function

    The point spread function (PSF) describes the response of a focused optical imaging system to a point source or point object. A more general term for the PSF is the system's impulse response; the PSF is the impulse response or impulse response function (IRF) of a focused optical imaging system. The PSF in many contexts can be thought of as the ...

  7. Objective (optics) - Wikipedia

    en.wikipedia.org/wiki/Objective_(optics)

    Two Leica oil immersion microscope objective lenses; left 100×, right 40×. The objective lens of a microscope is the one at the bottom near the sample. At its simplest, it is a very high-powered magnifying glass, with very short focal length. This is brought very close to the specimen being examined so that the light from the specimen comes ...

  8. Optical microscope - Wikipedia

    en.wikipedia.org/wiki/Optical_microscope

    The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.

  9. Two-photon excitation microscopy - Wikipedia

    en.wikipedia.org/wiki/Two-photon_excitation...

    Two-photon excitation microscopy of mouse intestine.Red: actin.Green: cell nuclei.Blue: mucus of goblet cells.Obtained at 780 nm using a Ti-sapphire laser.. Two-photon excitation microscopy (TPEF or 2PEF) is a fluorescence imaging technique that is particularly well-suited to image scattering living tissue of up to about one millimeter in thickness.