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Fluorescence microscopy relies upon fluorescent compounds, or fluorophores, in order to image biological systems.Since fluorescence and phosphorescence are competitive methods of relaxation, a fluorophore that undergoes intersystem crossing to the triplet excited state no longer fluoresces and instead remains in the triplet excited state, which has a relatively long lifetime, before ...
Jablonski diagram indicating intersystem crossing (left) and internal conversion (right). Internal conversion is a transition from a higher to a lower electronic state in a molecule or atom. [ 1 ] It is sometimes called "radiationless de-excitation", because no photons are emitted.
A Jablonski diagram showing the excitation of molecule A to its singlet excited state (1 A*) followed by intersystem crossing to the triplet state (3 A) that relaxes to the ground state by phosphorescence. It was used to describe absorption and emission of light by fluorescents.
Singlet fission is a spin-allowed process, unique to molecular photophysics, whereby one singlet excited state is converted into two triplet states.The phenomenon has been observed in molecular crystals, aggregates, disordered thin films, and covalently-linked dimers, where the chromophores are oriented such that the electronic coupling between singlet and the double triplet states is large.
Triplet states typically have longer lifetimes than excited singlets. The prolonged lifetime increases the probability of interacting with other molecules nearby. Photosensitizers experience varying levels of efficiency for intersystem crossing at different wavelengths of light based on the internal electronic structure of the molecule. [2] [7]
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Từ điển bách khoa toàn thư Việt Nam (Encyclopedia of Vietnam), a state-sponsored encyclopedia which was published in 2005. Vietnamese Wikipedia, a project of the Wikimedia Foundation. Vietnam War encyclopedias. Encyclopedic works and encyclopedias focused on Vietnam War-related topics.
Entrance sign at the tunnels. Part of the tunnel complex at Củ Chu, this tunnel has been made wider and taller to accommodate tourists. The tunnels of Củ Chi (Vietnamese: Địa đạo Củ Chi) are an immense network of connecting tunnels located in the Củ Chi District of Ho Chi Minh City (Saigon), Vietnam, and are part of a much larger network of tunnels that underlie much of the country.