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A 0.5 McFarland standard is prepared by mixing 0.05 mL of 1.175% barium chloride dihydrate (BaCl 2 •2H 2 O), with 9.95 mL of 1% sulfuric acid (H 2 SO 4). [ 1 ] Now there are McFarland standards prepared from suspensions of latex particles, which lengthens the shelf life and stability of the suspensions.
Test tube: Folin-Wu tube: Glass slide mycole and cover slips: in microscopy, serology, etc. as the solid backing on which test samples are Petri dish: used for preparation of culture media and the culture of organisms they are in Glass beaker: reagent storage Glass flask: gastric acid, or other fluid titration: Pasteur pipette
Sharps container [4] A imperforable container for sharp wastes like needles, blades, microscope slides, broken glass, etc. Cardboard biomedical waste containers: Reusable tubs [5] Colour coded biomedical waste bags (India) [6] - •Yellow plastic bags: for human anatomical, animal, microbiological and soiled waste •Red disinfected container ...
Once a bacterium has been identified following microbiological culture, antibiotics are selected for susceptibility testing. [5] Susceptibility testing methods are based on exposing bacteria to antibiotics and observing the effect on the growth of the bacteria (phenotypic testing), or identifying specific genetic markers (genetic testing). [6]
A biosafety cabinet (BSC)—also called a biological safety cabinet or microbiological safety cabinet—is an enclosed, ventilated laboratory workspace for safely working with materials contaminated with (or potentially contaminated with) pathogens requiring a defined biosafety level.
Bioburden is also associated with biofouling, where microbes collect on the surface of a device or inside of fan cooled equipment. In healthcare settings, this increases the risk of Healthcare-associated infections (HAIs) or Hospital-acquired infection as pathogens can be spread through contact or through the air to new patients and hospital staff.
used to inoculate test samples into culture media for bacterial or fungal cultures, antibiograms, etc. Sterilized by passing through a blue flame. Laminar flow cabinet: used to work aseptic Latex agglutination tiles: for serological analysis Lovibond comparator: a type of a colorimeter: McCartney's bottle or Flat medical bottle
Etest is a quantitative technique for determining the MIC of microoganisms. It is used for a range of Gram-negative and Gram-positive bacteria such as Pseudomonas, [2] [3] Staphylococcus, [4] and Enterococcus species, [5] as well as fastidious bacteria, such as Neisseria and Streptococcus pneumoniae. [1]