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The quinone is red-violet in colour, [3] [5] with the intensity of the colour being in proportion to the glucose concentration. [3] The colour is measured at 505 nm, [2] 510 nm, [4] or 540 nm. [3] Diagnostic kits containing the Trinder reagent are available, including one from Sigma-Aldrich. [2]
The glucose oxidase enzyme (GOx or GOD) also known as notatin (EC number 1.1.3.4) is an oxidoreductase that catalyses the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. This enzyme is produced by certain species of fungi and insects and displays antibacterial activity when oxygen and glucose are present.
3,3′,5,5′-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (). [1]
Sigma Advanced Genetic Engineering (SAGE) Labs is a division within Sigma-Aldrich that specializes in genetic manipulation of in vivo systems for special research and development applications. It was formed in 2008 to investigate zinc finger nuclease technology and its application for disease research models.
In biochemistry, ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) is a chemical compound used to observe the reaction kinetics of specific enzymes.A common use for it is in the enzyme-linked immunosorbent assay (ELISA) to detect the binding of molecules to each other.
The oxidase test is used to determine whether an organism possesses the cytochrome c oxidase enzyme. The test is used as an aid for the differentiation of Neisseria , Moraxella , Campylobacter and Pasteurella species (oxidase positive).
In enzymology, a glucose-fructose oxidoreductase (EC 1.1.99.28) is an enzyme that catalyzes the chemical reaction. D-glucose + D-fructose D-gluconolactone + D-glucitol. Thus, the two substrates of this enzyme are D-glucose and D-fructose, whereas its two products are D-gluconolactone and D-glucitol.
If the medium in the anaerobic tube turns yellow, then the bacteria are fermenting glucose. If the tube with oil doesn't turn yellow, but the open tube does turn yellow, then the bacterium is oxidizing glucose. If the tube with mineral oil doesn't change, and the open tube turns blue, then the organism neither ferments, nor oxidizes glucose.
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