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In microscopy, negative staining is an established method, often used in diagnostic microscopy, for contrasting a thin specimen with an optically opaque fluid. In this technique, the background is stained, leaving the actual specimen untouched, and thus visible. This contrasts with positive staining, in which the actual specimen is stained.
The negatively charged cell wall of many microorganisms attracts the positively charged chromophore which causes the specimen to absorb the stain giving it the color of the stain being used. Positive staining is more commonly used than negative staining in microbiology. The different types of positive staining are listed below. [1]
Rubber cups, also called prophy cups, are used in the hand-piece. Polishing paste, prophylactic paste, usually containing fluoride, is used with the rubber cups for polishing. Rubber cups should not be used over the cementum area as it may remove a layer of cementum at the cervical area. There are two popular types of prophy cups: 4 webs and 6 ...
ZOE can be used as a dental filling material or dental cement in dentistry. [ 1 ] [ 2 ] It is often used in dentistry when the decay is very deep or very close to the nerve or pulp chamber. Because the tissue inside the tooth, i.e. the pulp , reacts badly to the drilling stimulus (heat and vibration), it frequently becomes severely inflamed and ...
1. Isolate the tooth with a rubber dam 2. Perform a pulpotomy procedure. 3. Place the MTA material over the pulp and close the tooth with temporary cement until the apex is completely formed. MTA can be used in a one step or a two step approach. It can be used as a powder or a Wet Mix.
The bath consists of three containers filled with water at different temperatures: the first is set at 100 °C to liquefy the agar, the second is used to lower down the temperature of the material for safe intra-oral use (usually set at 43–46 °C) and the third one is used for storage and is set at 63–66 °C.
[1] More sophisticated varieties of plaque disclosing agents contain several dyes, which selectively stain plaque of different ages. With the most common variety, immature plaque stains red, mature purple, and pathological acidic plaque blue. This is owing to the blue dye washing off immature plaque, and acid degrading the red dye.
The Ziehl-Neelsen stain is a two step staining process. In the first step, the tissue is stained with a basic fuchsin solution, which stains all cells pink. In the second step, the tissue is incubated in an acid alcohol solution, which decolorizes all cells except for acid-fast cells, which retain the color and appeared as red.