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  2. Mannitol salt agar - Wikipedia

    en.wikipedia.org/wiki/Mannitol_salt_agar

    An MSA plate with Micrococcus sp. (1), Staphylococcus epidermidis (2) and S. aureus colonies (3). Mannitol salt agar or MSA is a commonly used selective and differential growth medium in microbiology. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. [1]

  3. Heyndrickxia coagulans - Wikipedia

    en.wikipedia.org/wiki/Heyndrickxia_coagulans

    H. coagulans is a Gram-positive, catalase-positive, spore-forming, motile, facultative anaerobe rod that measures approximately 0.9 μm by 3.0 μm to 5.0 μm. It may appear Gram negative when entering the stationary phase of growth. The optimum temperature for growth is 50 °C (122 °F); the range of temperatures tolerated is 30–55 °C (86 ...

  4. Agar plate - Wikipedia

    en.wikipedia.org/wiki/Agar_plate

    TSA plates support growth of many semifastidious bacteria, including some species of Brucella, Corynebacterium, Listeria, Neisseria, and Vibrio. Xylose-lysine-deoxycholate agar is used for the culture of stool samples and contains two indicators. It is formulated to inhibit Gram-positive bacteria, while the growth of Gram-negative bacilli is ...

  5. Growth medium - Wikipedia

    en.wikipedia.org/wiki/Growth_medium

    An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]

  6. Gram stain - Wikipedia

    en.wikipedia.org/wiki/Gram_stain

    The large CV–I complexes become trapped within the gram-positive cell due to the multilayered nature of its peptidoglycan. [18] The decolorization step is critical and must be timed correctly; the crystal violet stain is removed from both gram-positive and negative cells if the decolorizing agent is left on too long (a matter of seconds). [19]

  7. Plate count agar - Wikipedia

    en.wikipedia.org/wiki/Plate_count_agar

    The pour plate technique is the typical technique used to prepare plate count agars. Here, the inoculum is added to the molten agar before pouring the plate. The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample.

  8. Staining - Wikipedia

    en.wikipedia.org/wiki/Staining

    Starch is a substance common to most plant cells and so a weak iodine solution will stain starch present in the cells. Iodine is one component in the staining technique known as Gram staining, used in microbiology. Used as a mordant in Gram's staining, iodine enhances the entrance of the dye through the pores present in the cell wall/membrane.

  9. Negative stain - Wikipedia

    en.wikipedia.org/wiki/Negative_stain

    The choice of negative stain in electron microscopy can be very important. An early study of plant viruses using negatively stained leaf dips from a diseased plant showed only spherical viruses with one stain and only rod-shaped viruses with another. The verified conclusion was that this plant suffered from a mixed infection by two separate ...

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