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Fluorescence in situ hybridization (FISH) is a laboratory method used to detect and locate a DNA sequence, often on a particular chromosome. [4]In the 1960s, researchers Joseph Gall and Mary Lou Pardue found that molecular hybridization could be used to identify the position of DNA sequences in situ (i.e., in their natural positions within a chromosome).
A mule is a sterile hybrid of a male donkey and a female horse.Mules are smaller than horses but stronger than donkeys, making them useful as pack animals.. In biology, a hybrid is the offspring resulting from combining the qualities of two organisms of different varieties, subspecies, species or genera through sexual reproduction.
Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only particular parts of a nucleic acid sequence with a high degree of sequence complementarity.
For a hybrid form to persist, it must be able to exploit the available resources better than either parent species, which, in most cases, it will have to compete with.For example: while grizzly bears and polar bears may be able to mate and produce offspring, a grizzly–polar bear hybrid is apparently less- suited in either of the parents' ecological niches than the original parent species ...
In genomics, DNA–DNA hybridization is a molecular biology technique that measures the degree of genetic similarity between DNA sequences. It is used to determine the genetic distance between two organisms and has been used extensively in phylogeny and taxonomy .
A hybrid zone may appear during secondary contact, meaning there would be an area where the two populations cohabitate and produce hybrids, often arranged in a cline. The width of the zone may vary from tens of meters to several hundred kilometers. A hybrid zone may be stable, or it may not.
In molecular biology, a hybridization probe (HP) is a fragment of DNA or RNA, usually 15–10000 nucleotides long, which can be radioactively or fluorescently labeled. HPs can be used to detect the presence of nucleotide sequences in analyzed RNA or DNA that are complementary to the sequence in the probe. [ 1 ]
Sequencing by hybridization is a class of methods for determining the order in which nucleotides occur on a strand of DNA. Typically used for looking for small changes relative to a known DNA sequence . [ 1 ]