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Xylose lysine deoxycholate agar (XLD agar) is a selective growth medium used in the isolation of Salmonella and Shigella species from clinical samples and from food. [ 1 ] [ 2 ] The agar was developed by Welton Taylor in 1965. [ 3 ]
As there are many bacteria that also look like Salmonella on DCA, it is widely recommended that more selective agars are used for the identification of Salmonella, namely xylose lysine deoxycholate (XLD) agar. This growth medium is heat-sensitive and should be poured and cooled as soon as possible after addition of the deoxycholate, otherwise ...
Welton Ivan Taylor (November 12, 1919 – November 1, 2012) was an American microbiologist, inventor and civil rights activist.He is known for his work on food-borne pathogens, notably for developing tests for Salmonella and for inventing the XLD (Xylose Lysine Deoxycholate) agar, which can be used to isolate Salmonella and Shigella bacteria.
Xylose lysine deoxycholate or XLD agar, a growth medium for bacterial cultures; The XLD connector, a keyed variant of the XLR connector; An old Microsoft Excel file ...
Xylose-lysine-deoxycholate agar is used for the culture of stool samples and contains two indicators. It is formulated to inhibit Gram-positive bacteria, while the growth of Gram-negative bacilli is encouraged. The colonies of lactose fermenters appear yellow.
XLT Agar (Xylose Lysine Tergitol-4) is a selective culture medium for the isolation and identification of salmonellae from food and environmental samples. It is similar to XLD agar ; however, the agar is supplemented with the surfactant, Tergitol 4 , which causes inhibition of Proteus spp. and other non-Salmonellae. [ 1 ]
There is general agreement that almost 100% of Hafnia strains grow on MacConkey, Hektoen enteric, eosin methylene blue, and xylose-lysine-deoxycholate agars, all of which are differential to moderately selective media. [22]
It is urease and indole-negative. It produces acid oxidatively from xylose, but not from lactose, maltose, mannitol, or sucrose. It grows well on MacConkey agar and other inhibitory growth media such as deoxycholate, Salmonella-Shigella, and nalidixic acid-cetrimide agars. [3] [7] [8]