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The agar itself will turn red due to the presence of Salmonella type colonies. Shigella species: red colonies. Coliforms: yellow to orange colonies. Pseudomonas aeruginosa: pink, flat, rough colonies. This type of colony can be easily mistaken for Salmonella due to the color similarities. XLD agar contains:
TSI agar slant results: (from left) preinoculated (as control), P. aeruginosa, E. coli, Salmonella Typhimurium, Shigella flexneri The Triple Sugar Iron (TSI) test is a microbiological test roughly named for its ability to test a microorganism's ability to ferment sugars and to produce hydrogen sulfide. [1]
XLT Agar (Xylose Lysine Tergitol-4) is a selective culture medium for the isolation and identification of salmonellae from food and environmental samples. It is similar to XLD agar ; however, the agar is supplemented with the surfactant, Tergitol 4 , which causes inhibition of Proteus spp. and other non-Salmonellae. [ 1 ]
Önöz agar allows more rapid bacteriological diagnosis, as Salmonella and Shigella colonies can be clearly and reliably differentiated from other Enterobacteriaceae. The yields of Salmonella from stool samples obtained, when using this medium, are higher than those obtained with LEIFSON agar or Salmonella–Shigella agar.
Hektoen enteric agar (HEK, HE or HEA) is a selective and differential agar [1] primarily used to recover Salmonella and Shigella from patient specimens. HEA contains indicators of lactose fermentation and hydrogen sulfide production; as well as inhibitors to prevent the growth of Gram-positive bacteria .
After 24 hours of growth, this image depicts four different agar media culture plates that had been inoculated with Shigella sp., Escherichia sp., and Proteus sp. bacteria, (clockwise: MacConkey, Shigella-Salmonella, Bismuth Sulfite, and Brilliant Green agars). Bismuth sulfite agar is a type of agar media used to isolate Salmonella species.
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The term is usually restricted to Shigella infections. [2] Shigellosis is caused by one of several types of Shigella bacteria. [3] Three species are associated with bacillary dysentery: Shigella sonnei, Shigella flexneri and Shigella dysenteriae. [4] A study in China indicated that Shigella flexneri 2a was the most common serotype. [5]