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  2. Molar absorption coefficient - Wikipedia

    en.wikipedia.org/wiki/Molar_absorption_coefficient

    The SI unit of molar absorption coefficient is the square metre per mole (m 2 /mol), but in practice, quantities are usually expressed in terms of M −1 ⋅cm −1 or L⋅mol −1 ⋅cm −1 (the latter two units are both equal to 0.1 m 2 /mol). In older literature, the cm 2 /mol is sometimes used; 1 M −1 ⋅cm −1 equals 1000 cm 2 /mol.

  3. Absorbance - Wikipedia

    en.wikipedia.org/wiki/Absorbance

    Nevertheless, the absorbance unit or AU is commonly used in ultraviolet–visible spectroscopy and its high-performance liquid chromatography applications, often in derived units such as the milli-absorbance unit (mAU) or milli-absorbance unit-minutes (mAU×min), a unit of absorbance integrated over time. [6] Absorbance is related to optical ...

  4. Ultraviolet–visible spectroscopy - Wikipedia

    en.wikipedia.org/wiki/Ultraviolet–visible...

    where A is the measured absorbance (formally dimensionless but generally reported in absorbance units (AU) [12]), is the intensity of the incident light at a given wavelength, is the transmitted intensity, L the path length through the sample, and c the concentration of the absorbing

  5. Molar concentration - Wikipedia

    en.wikipedia.org/wiki/Molar_concentration

    Molar concentration or molarity is most commonly expressed in units of moles of solute per litre of solution. [2] For use in broader applications, it is defined as amount of substance of solute per unit volume of solution, or per unit volume available to the species, represented by lowercase : [3]

  6. Beer–Lambert law - Wikipedia

    en.wikipedia.org/wiki/Beer–Lambert_law

    Absorbance within range of 0.2 to 0.5 is ideal to maintain linearity in the Beer–Lambert law. If the radiation is especially intense, nonlinear optical processes can also cause variances. The main reason, however, is that the concentration dependence is in general non-linear and Beer's law is valid only under certain conditions as shown by ...

  7. Nucleic acid quantitation - Wikipedia

    en.wikipedia.org/wiki/Nucleic_acid_quantitation

    The "A260 unit" is used as a quantity measure for nucleic acids. One A260 unit is the amount of nucleic acid contained in 1 mL and producing an OD of 1. The same conversion factors apply, and therefore, in such contexts: 1 A260 unit dsDNA = 50 μg 1 A260 unit ssDNA = 33 μg 1 A260 unit ssRNA = 40 μg

  8. Concentration - Wikipedia

    en.wikipedia.org/wiki/Concentration

    In chemistry, concentration is the abundance of a constituent divided by the total volume of a mixture. Several types of mathematical description can be distinguished: mass concentration , molar concentration , number concentration , and volume concentration . [ 1 ]

  9. Colorimetry (chemical method) - Wikipedia

    en.wikipedia.org/wiki/Colorimetry_(chemical_method)

    A colorimeter is a device used to test the concentration of a solution by measuring its absorbance of a specific wavelength of light. To use this device, different solutions must be made, and a control (usually a mixture of distilled water and another solution) is first filled into a cuvette and placed inside a colorimeter to calibrate the machine.