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When the colonies are too numerous, it is common practice to count CFUs only on a fraction of the dish. Counting colonies is traditionally performed manually using a pen and a click-counter. This is generally a straightforward task, but can become very laborious and time-consuming when many plates have to be enumerated.
The Miles and Misra Method (or surface viable count) is a technique used in Microbiology to determine the number of colony forming units in a bacterial suspension or homogenate. The technique was first described in 1938 by Miles, Misra and Irwin who at the time were working at the LSHTM. [1] The Miles and Misra method has been shown to be ...
It is described using terms like brittle, creamy, sticky and dry. Staphylococci are considered to have a creamy consistency, [1]: 173 while some Neisseria species are sticky, and colonies of diphtheroid bacteria and beta-hemolytic streptococci are typically dry. [1]: 167–8 Bacteria that produce capsules often have a slimy (mucoid) consistency.
For instance, the bacterial colony is a cluster of identical cells (clones). These colonies often form and grow on the surface of (or within) a solid medium, usually derived from a single parent cell. [2] Colonies, in the context of development, may be composed of two or more unitary (or solitary) organisms or be modular organisms.
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The measurement of an exponential bacterial growth curve in batch culture was traditionally a part of the training of all microbiologists; the basic means requires bacterial enumeration (cell counting) by direct and individual (microscopic, flow cytometry [1]), direct and bulk (biomass), indirect and individual (colony counting), or indirect ...
Once a plate has been successfully prepared, plate count agar cells will grow into colonies which can be sufficiently isolated to determine the original cell type. The colony-forming unit (CFU) is an appropriate description of the colony's origin. In plate counts, colonies are counted, but the count is usually recorded in CFU.
The count represents the number of colony forming units (cfu) per g (or per ml) of the sample. A TVC is achieved by plating serial tenfold dilutions of the sample until between 30 and 300 colonies can be counted on a single plate. The reported count is the number of colonies counted multiplied by the dilution used for the counted plate