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  2. Flow cytometry - Wikipedia

    en.wikipedia.org/wiki/Flow_cytometry

    Flow cytometry (FC) is a technique used to detect and measure the physical and chemical characteristics of a population of cells or particles. [1] [2] [3] [4]In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument.

  3. Flow cytometry bioinformatics - Wikipedia

    en.wikipedia.org/wiki/Flow_cytometry_bioinformatics

    Flow cytometry bioinformatics requires extensive use of and contributes to the development of techniques from computational statistics and machine learning. Flow cytometry and related methods allow the quantification of multiple independent biomarkers on large numbers of single cells. The rapid growth in the multidimensionality and throughput ...

  4. Cell counting - Wikipedia

    en.wikipedia.org/wiki/Cell_counting

    Flow cytometry is by far the most sophisticated and expensive method for cell counting. In a flow cytometer the cells flow in a narrow stream in front of a laser beam. The beam hits them one by one, and a light detector picks up the light that is reflected from the cells.

  5. Cell cycle analysis - Wikipedia

    en.wikipedia.org/wiki/Cell_cycle_analysis

    Cell cycle analysis by DNA content measurement is a method that most frequently employs flow cytometry to distinguish cells in different phases of the cell cycle.Before analysis, the cells are usually permeabilised and treated with a fluorescent dye that stains DNA quantitatively, such as propidium iodide (PI) or 4,6-diamidino-2-phenylindole (DAPI).

  6. Pig-a gene mutation assay - Wikipedia

    en.wikipedia.org/wiki/Pig-a_gene_mutation_assay

    An in vivo RBC Pig-a assay is an optional test for qualification of genotoxic impurities as described in FDA's ICH M7 guidance. [3] A standardized regulatory-compliant Test Guideline for performing Pig-a assay and interpreting the test results is being developed under the auspices of The Organisation for Economic Co-operation and Development. [4]

  7. High-content screening - Wikipedia

    en.wikipedia.org/wiki/High-content_screening

    High-content screening technology is mainly based on automated digital microscopy and flow cytometry, in combination with IT-systems for the analysis and storage of the data. “High-content” or visual biology technology has two purposes, first to acquire spatially or temporally resolved information on an event and second to automatically ...

  8. Tetramer assay - Wikipedia

    en.wikipedia.org/wiki/Tetramer_assay

    The tetramers are labeled with a fluorophore, allowing tetramer-bound T-cells to be analyzed with flow cytometry. [4] Quantification and sorting of T-cells by flow cytometry enables researchers to investigate immune response to viral infection and vaccine administration as well as functionality of antigen-specific T-cells. [5]

  9. FlowJo - Wikipedia

    en.wikipedia.org/wiki/FlowJo

    FlowJo is a software package for analyzing flow cytometry data. Files produced by modern flow cytometers are written in the Flow Cytometry Standard [ 1 ] format with an .fcs file extension. FlowJo will import and analyze cytometry data regardless of which flow cytometer is used to collect the data.

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