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These hybrid DNA molecules can be then cleaved at the regenerated PstI sites. Its use is not limited to molecular cloning; it is also used in restriction site mapping, genotyping, Southern blotting, restriction fragment length polymorphism (RFLP) and SNP. [9] It is also an isoschizomer restriction enzyme SalPI from Streptomyces albus P. [10]
Cosmids are plasmids that incorporate a segment of bacteriophage λ DNA that has the cohesive end site (cos) which contains elements required for packaging DNA into λ particles. Under apt origin of replication (ori), it can replicate as a plasmid.
Cos sequences are ~200 base pairs long and essential for packaging. They contain a cosN site where DNA is nicked at each strand, 12 bp apart, by terminase.This causes linearization of the circular cosmid with two "cohesive" or "sticky ends" of 12bp.
The United States Food and Drugs Administration is warning pet owners about a common medication given to pets to treat arthritis. The F.D.A. now says that the drug Librela may be associated with ...
A Texas father is accused of intentionally setting his home on fire with his three children inside, according to police.. Pedro Luis Parra Pulgar, 46, is charged with three counts of attempted ...
President-elect Donald Trump had not been terribly successful in suing media organizations until this weekend when ABC News agreed to settle a closely-watched defamation case he brought against ...
It also provides easier development capability for the system suppliers at each end site. All pathology tests and profiles are coded from the Read Codes, a clinical terminology originally developed by a General Practitioner, James Read, to describe all aspects of healthcare for his own use but subsequently adopted and further developed by the ...
Simultaneously the donor strand is ligated to the target strand after cleavage leaving a single strand overhang on either end of the target sequence. These sites usually contain a 5 to 9 base pair overhang that can create a cohesive end. [10] Transposase then holds the sequence in a crossed formation and ligates the donor strand to the target ...