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Classical patch clamp setup, with microscope, antivibration table, and micromanipulators. During a patch clamp recording, a hollow glass tube known as a micropipette or patch pipette filled with an electrolyte solution and a recording electrode connected to an amplifier is brought into contact with the membrane of an isolated cell.
Two types of clamp are quite commonly used. The hyperglycemic clamp, which requires maintaining a high blood sugar level by perfusion or infusion with glucose, is a way to quantify how fast beta-cells respond to glucose. The hyperinsulinemic clamp, which requires maintaining a high insulin level by perfusion or infusion with insulin, is a way ...
The patch pipette is designed for whole cell recording so its opening diameter is larger than experiments done to examine single ion-channels. For the most part standard patch clamp protocols may be used although there are some small situation dependent modifications to the pipette and the internal solution.
A schematic of a patch clamp chip showing a gigaseal, whole cell recording configuration, and the ion channel and whole cell currents. Many types of systems have been developed for patch clamping cells in suspension cultures. One system uses a traditional pipette and cells in a droplet suspension culture to obtain patch clamp recordings (see ...
Cell isolation is the process of separating individual living cells from a solid block of tissue or cell suspension. While some types of cell naturally exist in a separated form (for example blood cells ), other cell types that are found in solid tissue require specific techniques to separate them into individual cells.
Fig 1. Channelrhodopsin-2 (ChR2) induces temporally precise blue light-driven activity in rat prelimbic prefrontal cortical neurons. a) In vitro schematic (left) showing blue light delivery and whole-cell patch-clamp recording of light-evoked activity from a fluorescent CaMKllα::ChR2-EYFP expressing pyramidal neuron (right) in an acute brain slice.
Bull Run Festival of Lights in Centreville, VA. Get ready to light up your holidays with this 2.5-mile drive-through extravaganza! Take a ride bursting with festive scenes, glowing characters, and ...
This page was last edited on 26 January 2012, at 15:04 (UTC).; Text is available under the Creative Commons Attribution-ShareAlike 4.0 License; additional terms may apply.
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