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COS-7 cells, 40X magnification. COS are fibroblast-like cell lines derived from monkey kidney tissue. COS cells are obtained by immortalizing CV-1 cells [1] with a version of the SV40 virus that can produce large T antigen but has a defect in genomic replication. [2] The CV-1 cell line in turn was derived from the kidney of the African green ...
The large CV–I complexes become trapped within the gram-positive cell due to the multilayered nature of its peptidoglycan. [18] The decolorization step is critical and must be timed correctly; the crystal violet stain is removed from both gram-positive and negative cells if the decolorizing agent is left on too long (a matter of seconds).
A Dictyostelium discoideum (slime mold) cell exhibiting a prominent contractile vacuole on its left side. The way in which water enters the CV had been a mystery for many years, but several discoveries since the 1990s have improved understanding of this issue.
The WI-38 cell line stemmed from earlier work by Hayflick growing human cell cultures. [2]In the early 1960s, Hayflick and his colleague Paul Moorhead at the Wistar Institute in Philadelphia, Pennsylvania discovered that when normal human cells were stored in a freezer, the cells remembered the doubling level at which they were stored and, when reconstituted, began to divide from that level to ...
The sustained expression of VCAM-1 lasts over 24 hours. Primarily, the VCAM-1 protein is an endothelial ligand for VLA-4 (Very Late Antigen-4 or integrin α4β1) of the β1 subfamily of integrins. VCAM-1 expression has also been observed in other cell types (e.g., smooth muscle cells). It has also been shown to interact with EZR [7] and Moesin. [7]
Many redox processes observed by CV are quasi-reversible or non-reversible. In such cases the thermodynamic potential E 0 1/2 is often deduced by simulation. The irreversibility is indicated by i pa /i pc ≠ 1. Deviations from unity are attributable to a subsequent chemical reaction that is triggered by the electron transfer
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Vero F6 is a cell transfected with the gene encoding HHV-1 entry protein glycoprotein-H (gH). [8] Vero F6 was transfected via a concatenated plasmid with the gH gene after a copy of the HHV-1 glycoprotein-D (gD) promoter region. In Vero lineage F6, expression of gH is under the control of the promoter region of gD. (Also F6B2; obs. F6B1.1)