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  2. Reverse Transcription Loop-mediated Isothermal Amplification

    en.wikipedia.org/wiki/Reverse_Transcription_Loop...

    Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a one step nucleic acid amplification method to multiply specific sequences of RNA. It is used to diagnose infectious disease caused by RNA viruses. [1] It combines LAMP [2] DNA-detection with reverse transcription, making cDNA from RNA before running the reaction. [3]

  3. Loop-mediated isothermal amplification - Wikipedia

    en.wikipedia.org/wiki/Loop-mediated_isothermal...

    Loop-mediated isothermal amplification (LAMP) primers [1] Loop-mediated isothermal amplification (LAMP) product [1]. In LAMP, the target sequence is amplified at a constant temperature of 60–65 °C (140–149 °F) using either two or three sets of primers and a polymerase like Bst Klenow fragment with high strand displacement activity in addition to a replication activity.

  4. Transcription-mediated amplification - Wikipedia

    en.wikipedia.org/wiki/Transcription-mediated...

    Transcription-mediated amplification (TMA) is an isothermal (performed at constant temperature), single-tube nucleic acid amplification system utilizing two enzymes, RNA polymerase and reverse transcriptase. "Amplification" means creating many more copies of a strand of nucleic acid than was present at first, in order to readily detect it or ...

  5. Retrotransposon - Wikipedia

    en.wikipedia.org/wiki/Retrotransposon

    Through reverse transcription, retrotransposons amplify themselves quickly to become abundant in eukaryotic genomes such as maize (49–78%) [3] and humans (42%). [4] They are only present in eukaryotes but share features with retroviruses such as HIV, for example, discontinuous reverse transcriptase-mediated extrachromosomal recombination. [5] [6]

  6. Recombinase polymerase amplification - Wikipedia

    en.wikipedia.org/wiki/Recombinase_Polymerase...

    By adding a reverse transcriptase enzyme to an RPA reaction, it can detect RNA as well as DNA, without the need for a separate step to produce cDNA. [2] [3] [4] Because it is isothermal, RPA can use much simpler equipment than PCR, which requires a thermal cycler. Operating best at temperatures of 37–42 °C and still working, albeit more ...

  7. Category:Molecular biology techniques - Wikipedia

    en.wikipedia.org/wiki/Category:Molecular_biology...

    Reverse complement polymerase chain reaction; Reverse northern blot; Reverse Transcription Loop-mediated Isothermal Amplification; Reverse transfection; Ribosomal intergenic spacer analysis; Ribosome profiling; RNase H-dependent PCR; Run-off transcription

  8. Rapid amplification of cDNA ends - Wikipedia

    en.wikipedia.org/wiki/Rapid_amplification_of...

    Rapid amplification of cDNA ends (RACE) is a technique used in molecular biology to obtain the full length sequence of an RNA transcript found within a cell. RACE results in the production of a cDNA copy of the RNA sequence of interest, produced through reverse transcription, followed by PCR amplification of the cDNA copies (see RT-PCR).

  9. Reverse transcriptase - Wikipedia

    en.wikipedia.org/wiki/Reverse_transcriptase

    A reverse transcriptase (RT) is an enzyme used to convert RNA genome to DNA, a process termed reverse transcription.Reverse transcriptases are used by viruses such as HIV and hepatitis B to replicate their genomes, by retrotransposon mobile genetic elements to proliferate within the host genome, and by eukaryotic cells to extend the telomeres at the ends of their linear chromosomes.

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