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Q-FISH. Quantitative Fluorescent in situ hybridization (Q-FISH) is a cytogenetic technique based on the traditional FISH methodology. In Q-FISH, the technique uses labelled (Cy3 or FITC) synthetic DNA mimics called peptide nucleic acid (PNA) oligonucleotides to quantify target sequences in chromosomal DNA using fluorescent microscopy and ...
A metaphase cell positive for the bcr/abl rearrangement (associated with chronic myelogenous leukemia) using FISH. The chromosomes can be seen in blue. The chromosome that is labeled with green and red spots (upper left) is the one where the rearrangement is present. Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique ...
hybridization. Chromogenic in situ hybridization (CISH) is a cytogenetic technique that combines the chromogenic signal detection method of immunohistochemistry (IHC) techniques with in situ hybridization. [1][2] It was developed around the year 2000 as an alternative to fluorescence in situ hybridization (FISH) for detection of HER-2/neu ...
In biochemistry, immunostaining is any use of an antibody -based method to detect a specific protein in a sample. The term "immunostaining" was originally used to refer to the immunohistochemical staining of tissue sections, as first described by Albert Coons in 1941. [1] However, immunostaining now encompasses a broad range of techniques used ...
The Division of Fish and Game was established in 1927, set up within the Department of Natural Resources. In 1951, the Reorganization Act elevated the Division of Fish and Game to the Department of Fish and Game (DFG). [1] California Fish and Game also collaborated with the indigenous Native American Tribes to ensure their proper fishing rights.
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Liu's stain (劉氏染色法) is a staining technique used to stain animal cells. It is an improved staining based on Romanowsky stain, and was introduced by professor Chen-Hui Liu(劉禎輝), faculty of National Taiwan University, in 1953. The method sees a wide variety of usage in Taiwan. Comparing to other staining methods, Liu's stain is ...
Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA. [1][2] These bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33,342nd compound made by the company. There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580.