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Also common in the microscopy literature is a formula for resolution that treats the above-mentioned concerns about contrast differently. [2] The resolution predicted by this formula is proportional to the Rayleigh-based formula, differing by about 20%. For estimating theoretical resolution, it may be adequate.
In optics, any optical instrument or system – a microscope, telescope, or camera – has a principal limit to its resolution due to the physics of diffraction. An optical instrument is said to be diffraction-limited if it has reached this limit of resolution performance. Other factors may affect an optical system's performance, such as lens ...
A 1951 USAF resolution test chart is a microscopic optical resolution test device originally defined by the U.S. Air Force MIL-STD-150A standard of 1951. The design provides numerous small target shapes exhibiting a stepped assortment of precise spatial frequency specimens. It is widely used in optical engineering laboratory work to analyze and ...
Angular resolution describes the ability of any image-forming device such as an optical or radio telescope, a microscope, a camera, or an eye, to distinguish small details of an object, thereby making it a major determinant of image resolution. It is used in optics applied to light waves, in antenna theory applied to radio waves, and in ...
Super-resolution optical fluctuation imaging ( SOFI) is a post-processing method for the calculation of super-resolved images from recorded image time series that is based on the temporal correlations of independently fluctuating fluorescent emitters. SOFI has been developed for super-resolution of biological specimen that are labelled with ...
In microscopy, NA generally refers to object-space numerical aperture unless otherwise noted. In microscopy, NA is important because it indicates the resolving power of a lens. The size of the finest detail that can be resolved (the resolution) is proportional to λ / 2NA , where λ is the wavelength of the light. A lens with a larger ...
The line-spread function is directly proportional to the vertical integration of the point-spread image. The optical-transfer function (OTF) is defined as the Fourier transform of the point-spread function and is thus generally a two-dimensional complex function. Typically only a one-dimensional slice is shown (c), corresponding to the Fourier ...
Optical sectioning is the process by which a suitably designed microscope can produce clear images of focal planes deep within a thick sample. This is used to reduce the need for thin sectioning using instruments such as the microtome. Many different techniques for optical sectioning are used and several microscopy techniques are specifically ...
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