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PCR food testing is the engagement of polymerase chain reaction (PCR) technologies for the testing of food for the presence or absence of human pathogens, such as E. coli, Salmonella, Listeria, [1] etc. [2] Four sample collection sites for PCR food testing can be: The food irrigation water. The food wash water.
Salmonella species can be found in the digestive tracts of humans and animals, especially reptiles. Salmonella on the skin of reptiles or amphibians can be passed to people who handle the animals. [39] Food and water can also be contaminated with the bacteria if they come in contact with the feces of infected people or animals. [40]
Food microbiology is the study of the microorganisms that inhabit, create, or contaminate food.This includes the study of microorganisms causing food spoilage; pathogens that may cause disease (especially if food is improperly cooked or stored); microbes used to produce fermented foods such as cheese, yogurt, bread, beer, and wine; and microbes with other useful roles, such as producing ...
Hektoen enteric agar (HEK, HE or HEA) is a selective and differential agar [1] primarily used to recover Salmonella and Shigella from patient specimens. HEA contains indicators of lactose fermentation and hydrogen sulfide production; as well as inhibitors to prevent the growth of Gram-positive bacteria.
Simmons’ citrate agar was developed by James S. Simmons in 1926 by adding 1.5% agar and bromothymol blue as a pH indicator to Koser’s citrate agar to observe changes in pH as a result of oxidative reactions from citrate metabolism. [5]
Additionally, the hurdle effect of food preservation, such as by adding lactic acid bacteria and salt to a food product, is illustrated and described. [ 11 ] [ 12 ] [ 13 ] This figure illustrates the pathway of food preservation followed by lactic acid bacteria involving nisin , as well as the pathway of food preservation followed by salt.
The Kauffmann–White classification or Kauffmann and White classification scheme [1] [2] is a system that classifies the genus Salmonella into serotypes, based on surface antigens. It is named after Philip Bruce White and Fritz Kauffmann . First the "O" antigen type is determined based on oligosaccharides associated with lipopolysaccharide.
For example, this study used data describing concentrations of Salmonella in chicken feces published in 1969. [14] Methods for quantifying bacteria, changes in animal housing practices and sanitation, and many other factors may have changed the prevalence of Salmonella since that time. Also, such an approach often ignores the complicated fate ...
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