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  2. Counterimmunoelectrophoresis - Wikipedia

    en.wikipedia.org/wiki/Counterimmunoelectrophoresis

    Plasmodium Glutamate dehydrogenase (pGluDH) separated by counterimmunoelectrophoresis [1]. Counterimmunoelectrophoresis is a laboratory technique used to evaluate the binding of an antibody to its antigen, it is similar to immunodiffusion, but with the addition of an applied electrical field across the diffusion medium, usually an agar or polyacrylamide gel.

  3. Immunoelectrophoresis - Wikipedia

    en.wikipedia.org/wiki/Immunoelectrophoresis

    Immunoelectrophoresis is a general term describing many combinations of the principles of electrophoresis and reaction of antibodies, also known as immunodiffusion. [1] Agarose as 1% gel slabs of about 1 mm thickness buffered at high pH (around 8.6) is traditionally preferred for electrophoresis and the reaction with antibodies. The agarose was ...

  4. SDS-PAGE - Wikipedia

    en.wikipedia.org/wiki/SDS-PAGE

    SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) is a discontinuous electrophoretic system developed by Ulrich K. Laemmli which is commonly used as a method to separate proteins with molecular masses between 5 and 250 kDa.

  5. Polyacrylamide gel electrophoresis - Wikipedia

    en.wikipedia.org/wiki/Polyacrylamide_gel...

    Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility. Electrophoretic mobility is a function of the length, conformation, and ...

  6. Immunoprecipitation - Wikipedia

    en.wikipedia.org/wiki/Immunoprecipitation

    Immunoprecipitation of intact protein complexes (i.e. antigen along with any proteins or ligands that are bound to it) is known as co-immunoprecipitation (Co-IP). Co-IP works by selecting an antibody that targets a known protein that is believed to be a member of a larger complex of proteins.

  7. Immunoproteomics - Wikipedia

    en.wikipedia.org/wiki/Immunoproteomics

    Two-dimensional gel electrophoresis (2-D gel) techniques in culmination with western blotting has been used for many years in the identification of immune response magnitude. [1] This can be accomplished by comparing various samples against molecular-weight size markers for qualitative analysis and against known amounts of protein standards for ...

  8. Immunohistochemistry - Wikipedia

    en.wikipedia.org/wiki/Immunohistochemistry

    Immunohistochemistry can be performed on tissue that has been fixed and embedded in paraffin, but also cryopreservated (frozen) tissue.Based on the way the tissue is preserved, there are different steps to prepare the tissue for immunohistochemistry, but the general method includes proper fixation, antigen retrieval incubation with primary antibody, then incubation with secondary antibody.

  9. Antibody-dependent enhancement - Wikipedia

    en.wikipedia.org/wiki/Antibody-dependent_enhancement

    In antibody-dependent enhancement, sub-optimal antibodies (the blue Y-shaped structures in the graphic) bind to both viruses and Fc gamma receptors (labeled FcγRII) expressed on immune cells, promoting infection of these cells.