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The blue–white screen is a screening technique that allows for the rapid and convenient detection of recombinant bacteria in vector-based molecular cloning experiments. This method of screening is usually performed using a suitable bacterial strain , but other organisms such as yeast may also be used.
DH5-Alpha Cells are E. coli cells engineered by American biologist Douglas Hanahan to maximize transformation efficiency. They are defined by three [1] mutations: recA1, endA1 which help plasmid insertion and lacZΔM15 which enables blue white screening.
This diagram was created with CorelDRAW. ... The following 2 pages use this file: Blue–white screen; ... some details may not fully reflect the modified file.
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A selectable marker is a gene introduced into cells, especially bacteria or cells in culture, which confers one or more traits suitable for artificial selection.They are a type of reporter gene used in laboratory microbiology, molecular biology, and genetic engineering to indicate the success of a transfection or transformation or other procedure meant to introduce foreign DNA into a cell.
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Diagramming in this context means creating and editing UML diagrams; that is diagrams that follow the graphical notation of the Unified Modeling Language. The use of UML diagrams as a means to draw diagrams of – mostly – object-oriented software is generally agreed upon by software developers. When developers draw diagrams of object ...
The N 2 chart or N 2 diagram (pronounced "en-two" or "en-squared") is a chart or diagram in the shape of a matrix, representing functional or physical interfaces between system elements. It is used to systematically identify, define, tabulate, design, and analyze functional and physical interfaces.