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A SNP array can also be used to generate a virtual karyotype using software to determine the copy number of each SNP on the array and then align the SNPs in chromosomal order. [10] SNPs can also be used to study genetic abnormalities in cancer. For example, SNP arrays can be used to study loss of heterozygosity (LOH). LOH occurs when one allele ...
Software suite to search and cluster huge sequence sets. Similar sensitivity to BLAST and PSI-BLAST but orders of magnitude faster: Protein: Steinegger M, Mirdita M, Galiez C, Söding J [10] 2017 USEARCH Ultra-fast sequence analysis tool: Both: Edgar, R. C. (2010). "Search and clustering orders of magnitude faster than BLAST". Bioinformatics.
Data analysis for an array-based DNA copy number test can be very challenging though due to very high volume of data that come out of an array platform. BAC (Bacterial Artificial Chromosome) arrays were historically the first microarray platform to be used for DNA copy number analysis. This platform is used to identify gross deletions or ...
There are three components that are critical to the KASP assay: 1) a purified DNA sample, 2) two allele-specific forward primers, and 3) a common reverse primer. A minimum of 5-10 ng of the extracted DNA sample is required for the method to function properly. The DNA sample is purified by adding a mixture of chemicals to the buffer solution. [2]
Phylogenetic inference and data visualization for allelic/SNP sequences profiles using Minimum Spanning Trees: All [29] SplitsTree: Software for viewing trees, cladograms, NeighborNets, and other graphs All [30] TreeDyn Open-source software for tree manipulation and annotation allowing incorporation of meta information: All [31] Treevolution
Since there is a massive number of SNPs on the genome, there is a clear need to prioritize SNPs according to their potential effect in order to expedite genotyping and analysis. [ 5 ] Annotating large numbers of SNPs is a difficult and complex process, which need computational methods to handle such a large dataset.
In order to perform SNP genotyping, Hardenbol et al. [3] modified padlock probes such that when the probe is hybridized to the genomic target, there is a gap at the SNP position. Gap filling using a nucleotide that is complementary to the nucleotide at the SNP location determines the identity of the polymorphism.
A tag SNP is a representative single nucleotide polymorphism (SNP) in a region of the genome with high linkage disequilibrium that represents a group of SNPs called a haplotype. It is possible to identify genetic variation and association to phenotypes without genotyping every SNP in a chromosomal region.