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The Ada regulon in E. coli is a well-characterized example of a group of genes involved in the adaptive response form of DNA repair. [6] Quorum sensing behavior in bacteria is a commonly cited example of a modulon or stimulon, [7] though some sources describe this type of intercellular auto-induction as a separate form of regulation. [1]
Once a bacterium has been identified following microbiological culture, antibiotics are selected for susceptibility testing. [5] Susceptibility testing methods are based on exposing bacteria to antibiotics and observing the effect on the growth of the bacteria (phenotypic testing), or identifying specific genetic markers (genetic testing). [6]
The Nif regulon is a set of seven operons used to regulate nitrogen fixation in the coliform bacterium Klebsiella pneumoniae under anaerobic and microaerophilic conditions. [1] It includes 17 nif genes , and is situated between the his and the Shi-A operon of the bacterium.
The yeast two-hybrid and bacterial two-hybrid screen [42] investigate the interaction between artificial fusion proteins. They do not require isolation of proteins but rather use transformation to express proteins in bacteria or yeast. The cells are designed in a way that an interaction activates the transcription of a reporter gene or a ...
This set of predicted binding sites corresponds to 12,574 TF → gene regulatory interactions; this represents a recovery of 52% of the 1592 annotated regulatory interactions in the database for the 93 TFs for which RegulonDB has a position-weight matrix (PWM). If only TFs with a good-quality PWM are taken into account, the total number of ...
A ligand binding assay (LBA) is an assay, or an analytic procedure, which relies on the binding of ligand molecules to receptors, antibodies or other macromolecules. [1] A detection method is used to determine the presence and amount of the ligand-receptor complexes formed, and this is usually determined electrochemically or through a fluorescence detection method. [2]
Two Indian chemical companies have been indicted for allegedly importing ingredients for the highly addictive opioid fentanyl into the United States and Mexico, the U.S. Department of Justice said ...
The Ada protein activates the transcription of the Ada regulon in two different ways. In case of the ada-alkB operon, and the aidB promoter, the N-terminal domain (AdaNTD) is involved in DNA binding and interacts with the a unit of RNA polymerase, whereas and the methylated C-terminal domain (me-AdaCTD) interacts with the σ 70 subunit of RNA ...