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Toluidine blue is also commonly used to stain frozen sections (rapid microscopic analysis of a specimen). Because time is of the essence for a frozen section, toluidine blue allows for the frozen section to be stained and reviewed in 10 to 20 seconds. [4] The other staining method for frozen sections (rapid H&E) takes approximately 60 to 90 ...
Hyaline cartilage coloured with the toluidine blue, in wich you can observe a strong metachromasia of the ground substance. View through optical microscope, 40x magnification. Metachromasia (var. metachromasy ) is a characteristic change in the color of staining carried out in biological tissues , exhibited by certain dyes when they bind to ...
Micromass cultures of C3H-10T1/2 cells at varied oxygen tensions stained with Alcian blue. Alcian blue (/ ˈ æ l ʃ ə n /) is any member of a family of polyvalent basic dyes, of which the Alcian blue 8G (also called Ingrain blue 1, and C.I. 74240, formerly called Alcian blue 8GX from the name of a batch of an ICI product) has been historically the most common and the most reliable member. [1]
Positive histologic stains that aid in the diagnosis of conditions of or affecting the human integumentary system Stain Cell, material, and/or structure(s) stained Condition(s) in which stain is positive Actin-specific enolase: Infantile digital fibromatosis: AE1/AE3: Squamous cell carcinoma: Alcian blue: Lipoid proteinosis Papular mucinosis ...
A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue .
Jaswant Singh–Bhattacharji stain, commonly referred to as JSB stain, is a rapid staining method for detection of malaria. [1] [2] It is useful for the diagnosis of malaria in thick smear samples of blood. [3] The JSB stain is commonly used throughout India, but rarely used in other countries. [4]
Toluidine blue is an example of a partially orthochromatic dye, as it stains nucleic acids by its orthochromatic color (blue), but stains mast cell granules in its metachromatic color (red). In spectral terms, orthochromasia refers to maintaining the position of spectral peaks, while metachromasia refers to a shift in wavelength, becoming ...
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