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433470 Ensembl ENSG00000166920 ENSMUSG00000033213 UniProt Q9C002 Q810Q5 RefSeq (mRNA) NM_197955 NM_032413 NM_001004174 RefSeq (protein) NP_115789 NP_922946 NP_115789.1 NP_922946.1 NP_001004174 Location (UCSC) Chr 15: 45.43 – 45.45 Mb Chr 2: 122.48 – 122.48 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Chromosome 15 open reading frame 48 is a protein that in humans is encoded by ...
The NMR sample is prepared in a thin-walled glass tube.. Protein nuclear magnetic resonance is performed on aqueous samples of highly purified protein. Usually, the sample consists of between 300 and 600 microlitres with a protein concentration in the range 0.1 – 3 millimolar.
Serum total protein, also known as total protein, is a clinical chemistry parameter representing the concentration of protein in serum. [1] Serum contains many proteins including serum albumin, a variety of globulins, and many others. While it is possible to analyze these proteins individually, total protein is a relatively quick and ...
Protein microarrays are rapid, automated, economical, and highly sensitive, consuming small quantities of samples and reagents. [4] The concept and methodology of protein microarrays was first introduced and illustrated in antibody microarrays (also referred to as antibody matrix) in 1983 in a scientific publication [5] and a series of patents. [6]
A mass spectrometer used for high throughput protein analysis. Protein mass spectrometry refers to the application of mass spectrometry to the study of proteins.Mass spectrometry is an important method for the accurate mass determination and characterization of proteins, and a variety of methods and instrumentations have been developed for its many uses.
Polyacrylamide gel electrophoresis is a powerful tool used to analyze RNA samples. When polyacrylamide gel is denatured after electrophoresis, it provides information on the sample composition of the RNA species. [1] Hydration of acrylonitrile results in formation of acrylamide molecules (C 3 H 5 NO) by nitrile hydratase. [2]
The analysis of the complete primary sequence of a protein using only one protease is usually not possible. In those cases the digestion of the target protein in several approaches with different enzymes is recommended. The resulting overlapping peptides permit the assembly of the complete sequence of the protein. [30] [36] [37]
The three samples are mixed and loaded onto IEF (isoelectric focusing chromatography) for first dimension and the strip is transferred to a SDS PAGE.After the gel electrophoresis, the gel is scanned with the excitation wavelength of each dye one after the other, so each sample can be seen separately (if we scan the gel at the excitation wavelength of the Cy3 dye, we will see in the gel only ...
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