Search results
Results from the WOW.Com Content Network
When using agar, within any growth medium, it is important to know that the solidification of the agar is pH-dependent. The optimal range for solidification is between 5.4 and 5.7. [43] Usually, the application of potassium hydroxide is needed to increase the pH to this range. A general guideline is about 600 μl 0.1M KOH per 250 ml GM.
Nutrient agar is a general-purpose solid medium supporting ... distilled water – water serves as a transport medium for the agar's various substances; pH adjusted ...
An agar plate is a Petri dish that contains a growth medium solidified with agar, used to culture microorganisms. ... lowering the pH and turning the plate yellow.
Sabouraud agar or Sabouraud dextrose agar (SDA) is a type of agar growth medium containing peptones. [1] It is used to cultivate dermatophytes and other types of fungi , and can also grow filamentous bacteria such as Nocardia .
However, once the agar has solidified, the pH does not appear to change with temperature but remains at 6.9. For preparation of BCYE + antibiotics, add membrane-filtered antibiotics and mix. For BCYE + albumin agar, dissolve the albumin in distilled water and filter sterilize before addition to the medium. Dispense 20 mL per 15 X 100-mm Petri dish.
De Man–Rogosa–Sharpe agar, often abbreviated to MRS, is a selective culture medium designed to favour the luxuriant growth of Lactobacilli for lab study. Developed in 1960, this medium was named for its inventors, Johannes Cornelis de Man [ Wikidata ] , Morrison Rogosa [ Wikidata ] , and Margaret Elisabeth Sharpe [ Wikidata ] .
Simmons’ citrate agar was developed by James S. Simmons in 1926 by adding 1.5% agar and bromothymol blue as a pH indicator to Koser’s citrate agar to observe changes in pH as a result of oxidative reactions from citrate metabolism. [5]
Mueller Hinton agar is a type of growth medium used in microbiology to culture bacterial isolates and test their susceptibility to antibiotics. This medium was first developed in 1941 by John Howard Mueller and Jane Hinton , who were microbiologists working at Harvard University.