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Differentiated adipocytes in a 3T3-L1 cell line stained with Oil Red O. 3T3-L1 is a sub clonal cell line derived from the original 3T3 Swiss albino cell line of 1962. The 3T3 original cell line was isolated from a mouse embryo and propagated for this specific line of 3T3 cells is used to study adipose tissue-related diseases and dysfunctions.
3T3 cells are several cell lines of mouse embryonic fibroblasts. The original 3T3 cell line (3T3-Swiss albino) was established in 1962 by two scientists then at the Department of Pathology in the New York University School of Medicine, George Todaro and Howard Green .
Reverse transfection is a technique for the transfer of genetic material into cells.As DNA is printed on a glass slide for the transfection process (the deliberate introduction of nucleic acids into cells) to occur before the addition of adherent cells, the order of addition of DNA and adherent cells is reverse that of conventional transfection. [1]
The PiggyBac (PB) transposon system employs a genetically engineered transposase enzyme to insert a gene into a cell's genome. It is built upon the natural PiggyBac (PB) transposable element (transposon), enabling the back and forth movement of genes between chromosomes and genetic vectors such as plasmids through a "cut and paste" mechanism.
Transfection is the process of deliberately introducing naked or purified nucleic acids into eukaryotic cells. [ 1 ] [ 2 ] It may also refer to other methods and cell types, although other terms are often preferred: " transformation " is typically used to describe non-viral DNA transfer in bacteria and non-animal eukaryotic cells, including ...
Tissue nanotransfection (TNT) is an electroporation-based technique capable of gene and drug cargo delivery or transfection at the nanoscale. Furthermore, TNT is a scaffold-less tissue engineering (TE) technique that can be considered cell-only or tissue inducing depending on cellular or tissue level applications.
OpenADR – Open Automated Demand Response; protocol to manage electricity consuming/controlling devices; Optomux – Serial (RS-422/485) network protocol originally developed by Opto 22 in 1982. The protocol was openly documented [1] and over time used for industrial automation applications. PieP – An Open Fieldbus Protocol
Protocols, however, exist for making supercompetent cells that may yield a transformation efficiency of over 10 9. [61] The chemical method, however, usually does not work well for linear DNA, such as fragments of chromosomal DNA, probably because the cell's native exonuclease enzymes rapidly degrade linear DNA.