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The complement fixation test is an immunological medical test that can be used to detect the presence of either specific antibody or specific antigen in a patient's serum, based on whether complement fixation occurs. It was widely used to diagnose infections, particularly with microbes that are not easily detected by culture methods, and in ...
Direct immunofluorescence can be used to detect deposits of immunoglobulins and complement proteins in biopsies of skin, kidney and other organs. Their presence is indicative of an autoimmune disease. When skin not exposed to the sun is tested, a positive direct IF (the so-called Lupus band test) is an evidence of systemic lupus erythematosus. [2]
"Chapter 12: Precipitation: Single Radial Immunodiffusion: Laboratory Technique 12-1: Radial Immunodiffusion Test". Essentials of Immunology & Serology. Albany, New York: Delmar Division of Thomson Learning. pp. 172– 174. ISBN 978-0914826255. LCCN 2002280630. OCLC 1149023866 – via Internet Archive.
Immunodiffusion is a laboratory technique used to detect and quantify antigens and antibodies by observing their interactions within a gel medium. [1] This technique involves the diffusion of antigens and antibodies through a gel, usually agar, resulting in the formation of a visible precipitate when they interact.
The Wassermann test or Wassermann reaction (WR) [1] is an antibody test for syphilis, named after the bacteriologist August Paul von Wassermann, based on complement fixation. It was the first blood test for syphilis and the first in the nontreponemal test (NTT) category. Newer NTTs, such as the RPR and VDRL tests, have mostly replaced it ...
Diagnostic tools to measure complement activity include the total complement activity test. [52] The presence or absence of complement fixation upon a challenge can indicate whether particular antigens or antibodies are present in the blood. This is the principle of the complement fixation test. [citation needed]
complement-fixing antibody colonization factor antigen CFIDS: chronic fatigue immune dysfunction syndrome: CFR: case fatality rate: CFS: chronic fatigue syndrome: CFT: complement fixation test capillary filling time: CFTR: cystic fibrosis transmembrane conductance regulator: CFU: colony-forming unit: CGD: chronic granulomatous disease: CGI
The test is based on the capacity of an individual's serum to lyse sheep erythrocytes coated with anti-sheep antibodies (preferably rabbit IgG). The individual's serum is diluted until a minimum concentration of 50% of the sheep's red blood cells are lysed.