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Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
A condenser (right) and its respective diaphragm (left) A condenser is an optical lens that renders a divergent light beam from a point light source into a parallel or converging beam to illuminate an object to be imaged. Condensers are an essential part of any imaging device, such as microscopes, enlargers, slide projectors, and telescopes.
Endomicroscopy. Endomicroscopy is a technique for obtaining histology -like images from inside the human body in real-time, [1] [2] [3] a process known as ‘optical biopsy’. [4] [5] It generally refers to fluorescence confocal microscopy, although multi-photon microscopy and optical coherence tomography have also been adapted for endoscopic use.
The lateral resolution of light sheet fluorescence microscopy can be improved beyond the Abbe limit, by using super resolution microscopy techniques, e.g. with using the fact, that single fluorophores can be located with much higher spatial precision than the nominal resolution of the used optical system (see stochastic localization microscopy ...
First workshop of Carl Zeiss in the center of Jena, c. 1847 Carl Zeiss Jena (1910) One of the Stasi's cameras with the special SO-3.5.1 (5/17mm) lens developed by Carl Zeiss, a so-called "needle eye lens", for shooting through keyholes or holes down to 1 mm in diameter 2 historical lenses of Carl Zeiss, Nr. 145077 and Nr. 145078, Tessar 1:4,5 F=5,5cm DRP 142294 (produced before 1910) Carl ...
The first optically feasible stereomicroscope was invented in 1892 and became commercially available in 1896, produced by Zeiss AG in Jena, Germany. [2] 1896 Greenough Stereo Microscope by Carl Zeiss Jena. American zoologist Horatio Saltonstall Greenough grew up in the elite of Boston, Massachusetts, the son of the famous sculptor Horatio ...
Scanning laser ophthalmoscopy (SLO) is a method of examination of the eye. It uses the technique of confocal laser scanning microscopy for diagnostic imaging of the retina or cornea of the human eye. As a method used to image the retina with a high degree of spatial sensitivity, it is helpful in the diagnosis of glaucoma, macular degeneration ...
Phase-contrast microscopy (PCM) is an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image. Phase shifts themselves are invisible, but become visible when shown as brightness variations. When light waves travel through a medium other than a vacuum, interaction ...
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