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Brushes used to collect samples for cytology. Liquid-based cytology is a method of preparing samples for examination in cytopathology.The sample is collected, normally by a small brush, in the same way as for a conventional smear test, but rather than the smear being transferred directly to a microscope slide, the sample is deposited into a small bottle of preservative liquid.
The third staining solution is composed of three dyes, Eosin Y, Light Green SF yellowish, and Bismarck brown Y in 95% ethyl alcohol with a small amount of phosphotungstic acid and lithium carbonate. [ 12 ] [ 2 ] This solution, designated EA, followed by a number that denotes the proportion of the dyes, other formulations include EA-36, EA-50 ...
Further information about the specimen may be gained by immunohistochemical stains and molecular testing, particularly if the sample is prepared using liquid based cytology. Often "reflex" testing is performed, such as HPV testing on an abnormal pap test or flow cytometry on a lymphoma specimen.
Giemsa's solution is a mixture of methylene blue, eosin, and Azure B. The stain is usually prepared from commercially available Giemsa powder. A thin film of the specimen on a microscope slide is fixed in pure methanol for 30 seconds, by immersing it or by putting a few drops of methanol on the slide. The slide is immersed in a freshly prepared ...
Heinz bodies are associated with the consumption of paracetamol (acetaminophen), garlic, [11] [12] and onions by cats, [13] dogs, and various primates. Thiosulfate compounds in the flesh of onions have been identified as the cause. Propylene glycol was once a common ingredient in soft moist cat food. According to the FDA "It was known for some ...
Fine-needle aspiration (FNA) is a diagnostic procedure used to investigate lumps or masses.In this technique, a thin (23–25 gauge (0.52 to 0.64 mm outer diameter)), hollow needle is inserted into the mass for sampling of cells that, after being stained, are examined under a microscope ().
Example of a cytocentrifuge. A cytocentrifuge, sometimes referred to as a cytospin, [1] is a specialized centrifuge used to concentrate cells in fluid specimens onto a microscope slide so that they can be stained and examined. [2]
A liquid biopsy, also known as fluid biopsy or fluid phase biopsy, is the sampling and analysis of non-solid biological tissue, primarily blood. [ 1 ] [ 2 ] Like traditional biopsy , this type of technique is mainly used as a diagnostic and monitoring tool for diseases such as cancer , with the added benefit of being largely non-invasive.