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DNA gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via polymerase chain reaction (PCR), but may be used as a preparative technique for other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or southern blotting for further characterization.
Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine. [2]
Cold Spring Harbor Protocols (formerly CSH Protocols) is an online scientific journal and methods database for biologists, published by Cold Spring Harbor Laboratory Press. Protocols are presented step-by-step and edited in the style that has made Molecular Cloning, Antibodies, Cells and many other CSH manuals essential [ tone ] to the work of ...
Cold Spring Harbor Laboratory (CSHL) is a private, non-profit institution with research programs focusing on cancer, neuroscience, plant biology, genomics, and quantitative biology. [2] It is located in Laurel Hollow on Long Island , New York.
The DNA Learning Center (DNALC) is a genetics learning center affiliated with the Cold Spring Harbor Laboratory, in Cold Spring Harbor, New York.It is the world's first science center devoted entirely to genetics education and offers online education, class field trips, student summer day camps, and teacher training.
The school was formerly named after James Watson, co-discoverer of the structure of DNA and then president of the laboratory, shortly after its founding.On July 1, 2020, the board of trustees voted to return the name of the school to the "Cold Spring Harbor Laboratory School of Biological Sciences" because of Watson's views on race.
TAE buffer is commonly prepared as a 50× stock solution for laboratory use. A 50× stock solution can be prepared by dissolving 242 g Tris base in water, adding 57.1 ml glacial acetic acid, and 100 ml of 500 mM EDTA (pH 8.0) solution, and bringing the final volume up to 1 litre.
DNA Isolation: The DNA to be studied is isolated from various tissues. The most suitable source of DNA is known as blood tissue. However, it can be isolated from different tissues (hair, semen, saliva, etc.). DNA digestion: Restriction endonucleases are used to cut high-molecular-weight DNA strands into smaller fragments. This is done by adding ...