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The ligase chain reaction (LCR) is a method of DNA amplification. The ligase chain reaction (LCR) is an amplification process that differs from polymerase chain reaction (PCR) in that it involves a thermostable ligase to join two probes or other molecules together which can then be amplified by standard PCR cycling. [ 1 ]
Furthermore, the union or intersection of the results of the search on a query sequence can be obtained. A Neural Network webserver, named LCR-hound has been developed to predict the function of prokaryotic and eukaryotic LCRs, based on their amino acid or di-amino acid content.
A locus control region (LCR) is a long-range cis-regulatory element that enhances expression of linked genes at distal chromatin sites. It functions in a copy number-dependent manner and is tissue-specific, as seen in the selective expression of β-globin genes in erythroid cells . [ 1 ]
Low complexity regions in proteins can be computationally detected from sequence using various methods and definitions, as reviewed in. [2] Among the most popular methodologies to identify LCRs is by measuring their Shannon entropy. [1] The lower the value of the calculated entropy, the more homogeneous the region is in terms of amino acid content.
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As it refers to the product of an amplification reaction, amplicon is used interchangeably with common laboratory terms, such as "PCR product." Artificial amplification is used in research , [ 1 ] forensics , [ 2 ] and medicine [ 1 ] for purposes that include detection and quantification of infectious agents , [ 3 ] identification of human ...
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