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These aspects of immunohistochemistry tissue prep and antibody staining must be systematically addressed to identify and overcome staining issues. [5] [6] Positive and negative controls in immunohistochemistry. For this purpose, "internal" means tissue from the target patient, and "external" means that the tissue is from another patient. [17]
The term "immunostaining" was originally used to refer to the immunohistochemical staining of tissue sections, as first described by Albert Coons in 1941. [1] However, immunostaining now encompasses a broad range of techniques used in histology, cell biology, and molecular biology that use antibody-based staining methods.
Positive histologic stains that aid in the diagnosis of conditions of or affecting the human integumentary system Stain Cell, material, and/or structure(s) stained Condition(s) in which stain is positive Actin-specific enolase: Infantile digital fibromatosis: AE1/AE3: Squamous cell carcinoma: Alcian blue: Lipoid proteinosis Papular mucinosis ...
Immunohistochemistry with cytokeratin 5/6 antibodies in usual ductal hyperplasia, showing a mosaic pattern, predominantly in the central zone. Cytokeratin 5/6 antibodies are antibodies that target both cytokeratin 5 and cytokeratin 6. [1] These are used in immunohistochemistry, often called CK 5/6 staining, [2] including the following applications:
20665 Ensembl ENSG00000100146 ENSMUSG00000033006 UniProt P56693 Q04888 RefSeq (mRNA) NM_006941 NM_011437 RefSeq (protein) NP_008872 NP_035567 Location (UCSC) Chr 22: 37.97 – 37.99 Mb Chr 15: 79.04 – 79.05 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Transcription factor SOX-10 is a protein that in humans is encoded by the SOX10 gene. Function This gene encodes a member of the ...
- The right panel shows high magnification on a positive area, confirming adenocarcinoma, as it shows tumor cells with large nuclei and prominent nucleoli. AE1/AE3 is an antibody cocktail that is used in immunohistochemistry , being generally positive in the cytoplasm of carcinomas (cancers of epithelial origin).
p16 is a widely used immunohistochemical marker in gynecologic pathology. Strong and diffuse cytoplasmic and nuclear expression of p16 in squamous cell carcinomas (SCC) of the female genital tract is strongly associated with high-risk human papilloma virus (HPV) infection and neoplasms of cervical origin.
Comparison H&E stain (left) with BerEP4 immunohistochemistry staining (right) on superficial BCC pathological sections mimicking Bowen’s Disease. [1] At bottom, columnar epithelium in normal sweat glands stain positive too. BerEp4 (also Ber-EP4) is a histologic stain mainly used to aid in the diagnosis of basal cell carcinoma (BCC). [2]
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