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S. cerevisiae septins revealed with fluorescent microscopy utilizing fluorescent labeling. In molecular biology and biotechnology, a fluorescent tag, also known as a fluorescent label or fluorescent probe, is a molecule that is attached chemically to aid in the detection of a biomolecule such as a protein, antibody, or amino acid.
Laser-induced fluorescence (LIF) or laser-stimulated fluorescence (LSF) [1] is a spectroscopic method in which an atom or molecule is excited to a higher energy level by the absorption of laser light followed by spontaneous emission of light. [2] [3] It was first reported by Zare and coworkers in 1968. [4] [5]
Consequently, the combination of labels which can be used depends on the wavelength of the lamp(s) or laser(s) used to excite the fluorochromes and on the detectors available. [37] Flow cytometry uses fluorescence as a quantitative tool; the utmost sensitivity of flow cytometry is unmatched by other fluorescent detection platforms such as ...
It is an amine-labeling dye that is not fluorescent itself, but reacts with primary amines to form fluorescent products. [2] It is within the "chameleon labels" class, [1] so named due to their clear color-changing properties upon conjugation. Py-1 was first reported in 2004. [3]
Some of the widely used laser sources are Nd:YAG laser, dye lasers, excimer lasers, and ion lasers. The light from the laser (usually a beam) is passed through a set of lenses and/or mirrors to form a sheet, which is then used to illuminate the medium. This medium is either made up of fluorescent material or can be seeded with a fluorescent ...
The DyLight Fluor family of fluorescent dyes are produced by Dyomics in collaboration with Thermo Fisher Scientific. [4] DyLight dyes are typically used in biotechnology and research applications as biomolecule, cell and tissue labels for fluorescence microscopy, cell biology or molecular biology.
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