Search results
Results from the WOW.Com Content Network
The last 10-12 bases at the 3' end of a primer are sensitive to initiation of polymerase extension and general primer stability on the template binding site. The effect of a single mismatch at these last 10 bases at the 3' end of the primer depends on its position and local structure, reducing the primer binding, selectivity, and PCR efficiency.
The free NCBI tool Primer-BLAST integrates primer design and BLAST search into one application, [6] as do commercial software products such as ePrime and Beacon Designer. Computer simulations of theoretical PCR results ( Electronic PCR ) may be performed to assist in primer design by giving melting and annealing temperatures, etc. [ 7 ]
For example, lower concentrations of magnesium or other cations may prevent non-specific primer interactions, thus enabling successful PCR. A "hot-start" polymerase enzyme whose activity is blocked unless it is heated to high temperature (e.g., 90–98˚C) during the denaturation step of the first cycle, is commonly used to prevent non-specific ...
Written by physicist Robert Serber, the Primer describes what was known in 1943 about what technical and scientific questions still remained to create a "practical military weapon" out of scientific principles discovered only four years before. It was declassified in the early 1960s, and an annotated version has been released by the University ...
A primer binding site is a region of a nucleotide sequence where an RNA or DNA single-stranded primer binds to start replication. The primer binding site is on one of the two complementary strands of a double-stranded nucleotide polymer , in the strand which is to be copied, or is within a single-stranded nucleotide polymer sequence.
A Republican senator has blocked the promotion of a general who oversaw the US withdrawal from Afghanistan, according to a source familiar with the matter, as President-elect Donald Trump has ...
Extra Meat, Extra Cheese, Extra Inflation. There are few restaurants of any variety as beloved as Chipotle in this country, and value is a big reason for a lot of people.
BLAST is more time-efficient than FASTA by searching only for the more significant patterns in the sequences, yet with comparative sensitivity. This could be further realized by understanding the algorithm of BLAST introduced below. Examples of other questions that researchers use BLAST to answer are: