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Chromatographic peak resolution is given by = + where t R is the retention time and w b is the peak width at baseline. The bigger the time-difference and/or the smaller the bandwidths, the better the resolution of the compounds. Here compound 1 elutes before compound 2.
3) Changing α is the most effective way of increasing resolution. This can be done by choosing a stationary phase that has a greater difference between k 1 ' and k 2 '. It can also be done in L.C. by using pH to invoke secondary equilibria (if applicable). The fundamental resolution equation is derived as follows:
From the variables in the figure above, the resolution, plate number, and plate height of the column plate model can be calculated using the equations: Resolution (R s): R s = 2(t RB – t RA)/(w B + w A), where: t RB = retention time of solute B t RA = retention time of solute A w B = Gaussian curve width of solute B w A = Gaussian curve width ...
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