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The method was first described in 2002 in the scientific journal Nucleic Acid Research. [2] The first applications included the detection of exon deletions in the human genes BRCA1, MSH2 and MLH1, which are linked to hereditary breast and colon cancer. Now MLPA is used to detect hundreds of hereditary disorders, as well as for tumour profiling.
Ploidy (/ ˈ p l ɔɪ d i /) is the number of complete sets of chromosomes in a cell, and hence the number of possible alleles for autosomal and pseudoautosomal genes. Here sets of chromosomes refers to the number of maternal and paternal chromosome copies, respectively, in each homologous chromosome pair—the form in which chromosomes ...
This is also the most common pathway of artificially induced polyploidy, where methods such as protoplast fusion or treatment with colchicine, oryzalin or mitotic inhibitors are used to disrupt normal mitotic division, which results in the production of polyploid cells. This process can be useful in plant breeding, especially when attempting to ...
Print/export Download as PDF ... Appearance. move to sidebar hide. Help. Laboratory methods and techniques, as used in ... Text is available under the Creative ...
Methods in Molecular Biology is a book series published by Humana Press (an imprint of Springer Science+Business Media) that covers molecular biology research methods and protocols. The book series was introduced by series editor John M. Walker in 1983 and provides step-by-step instructions for carrying out experiments in a research lab. [1]
A polyploid complex, also called a diploid-polyploid complex, is a group of interrelated and interbreeding species that also have differing levels of ploidy that can allow interbreeding. A polyploid complex was described by E. B. Babcock and G. Ledyard Stebbins in their 1938 monograph The American Species of Crepis : their interrelationships ...
Most modern aptamer selection methods strive to improve the conventional SELEX aptamer search method. [30] Despite the publication of various methods aimed at increasing the affinity and specificity of aptamers, [ 31 ] [ 32 ] [ 33 ] experimental approaches face limitations in the number and variety of sequences that can be examined and selected.
Types of mutations that can be introduced by random, site-directed, combinatorial, or insertional mutagenesis. In molecular biology, mutagenesis is an important laboratory technique whereby DNA mutations are deliberately engineered to produce libraries of mutant genes, proteins, strains of bacteria, or other genetically modified organisms.