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A germicidal lamp (also known as disinfection lamp or sterilizer lamp) is an electric light that produces ultraviolet C (UVC) light. This short-wave ultraviolet light disrupts DNA base pairing , causing formation of pyrimidine dimers , and leads to the inactivation of bacteria , viruses , and protozoans .
Ultraviolet germicidal irradiation (UVGI) is a disinfection technique employing ultraviolet (UV) light, particularly UV-C (180–280 nm), to kill or inactivate microorganisms. UVGI primarily inactivates microbes by damaging their genetic material, thereby inhibiting their capacity to carry out vital functions.
The location of 'far-UVC' radiation (200-235 nm) in the electromagnetic spectrum. Far-UVC is a type of ultraviolet germicidal irradiation being studied and commercially developed for its combination of pathogen inactivation properties and reduced negative effects on human health when used within exposure guidelines.
UVC LEDs are developing rapidly, but may require testing to verify effective disinfection. Citations for large-area disinfection are for non-LED UV sources [39] known as germicidal lamps. [40] Also, they are used as line sources to replace deuterium lamps in liquid chromatography instruments. [41]
Because UVC can be deadly, a special dichroic filter glass (usually purple) is required that will filter out the UVC and UVB. The goal with high-pressure tanning bulbs is to produce a high amount of UVA only. Unfiltered light from a high-pressure lamp is rich in UVC used in germicidal lamps, for water purification, but it damages human skin.
Germicidal UV-C light in the short wavelength range of 100–280 nm acts on thymine, one of the four base nucleotides in DNA. When a germicidal UV photon is absorbed by a thymine molecule that is adjacent to another thymine within the DNA strand, a covalent bond or dimer between the molecules is created.
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