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To make a 100 ml solution of T 10 E 1 buffer, 1 ml of 1 M Tris base (pH 10–11) and 0.2 ml EDTA (0.5 M) are mixed and made up with double distilled water up to 100ml. Add microliter amounts of high molarity HCl to lower the pH to 8. Based on nuclease studies from the 1980s, the pH is usually adjusted to 7.5 for RNA and 8.0 for DNA.
A 0.5 McFarland standard is prepared by mixing 0.05 mL of 1.175% barium chloride dihydrate (BaCl 2 •2H 2 O), with 9.95 mL of 1% sulfuric acid (H 2 SO 4). [ 1 ] Now there are McFarland standards prepared from suspensions of latex particles, which lengthens the shelf life and stability of the suspensions.
To create the solution, 11.6 g NaCl is placed in a volumetric flask, dissolved in some water, then followed by the addition of more water until the total volume reaches 100 mL. The density of water is approximately 1000 g/L and its molar mass is 18.02 g/mol (or 1/18.02 = 0.055 mol/g). Therefore, the molar concentration of water is
The sizes most commonly used are 1 mL, 2.5 mL, 3 mL, 5 mL and 10 mL. ... microliter syringes can be used to measure and dose chemicals very precisely by using a small ...
The sample cells can also be used for the measurement of poisonous and toxic samples with minimum exposure to the sample. Micro cells require only a few microliters volume, assure good recovery of expensive samples and prevent evaporation of volatile samples or solvents.
1 cm – edge of a cube of volume 1 mL; 1 cm – length of a coffee bean; 1 cm – approximate width of average fingernail; 1.2 cm – length of a bee; 1.2 cm – diameter of a die; 1.5 cm – length of a very large mosquito; 1.6 cm – length of a Jaragua Sphaero, a very small reptile; 1.7 cm – length of a Thorius arboreus, the smallest ...
For the experimental portion of the assay, cells are diluted in 10 mM sodium phosphate pH 7.4 such that the final cell concentration in 10 microliters is 5x10 6 CFUv/mL. 10 μl of this cell suspension are pipetted beneath the 90 μl of antimicrobial peptides in solution, resulting in a cell suspension at the standard inoculum of 5x10 5 CFUv/mL ...
Air displacement micropipettes are a type of adjustable micropipette that deliver a measured volume of liquid; depending on size, it could be between about 0.1 μL to 1,000 μL (1 mL). These pipettes require disposable tips that come in contact with the fluid. These pipettes operate by piston-driven air displacement. A vacuum is generated by ...