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If A549 cells are cultured in vitro, they grow as a monolayer; adherent or attaching to the culture flask. [1] The cells are able to synthesize lecithin and contain high levels of unsaturated fatty acids, which are important to maintain membrane phospholipids. [1] A549 cells are widely used as a type II pulmonary epithelial cell model for drug ...
Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use. Tissue engineering potentially offers ...
This page was last edited on 23 November 2019, at 17:55 (UTC).; Text is available under the Creative Commons Attribution-ShareAlike 4.0 License; additional terms may apply.
Cell cycle analysis by DNA content measurement is a method that most frequently employs flow cytometry to distinguish cells in different phases of the cell cycle.Before analysis, the cells are usually permeabilised and treated with a fluorescent dye that stains DNA quantitatively, such as propidium iodide (PI) or 4,6-diamidino-2-phenylindole (DAPI).
Mixed co-culture is the simplest co-culture method, where two types of cells are in direct contact within a single culture compartment at the desired cell ratio. [47] Cells can communicate by paracrine and juxtacrine signaling, but separated treatments and downstream analysis of a single cell type are not readily feasible due to the completely ...
The fluorochrome-based TUNEL assay applicable for flow cytometry, combining the detection of DNA strand breaks with respect to the cell cycle-phase position, was originally developed by Gorczyca et al. [4] Concurrently, the avidin-peroxidase labeling assay applicable for light absorption microscope was described by Gavrieli et al. [5] Since 1992 the TUNEL has become one of the main methods for ...
ATCC or the American Type Culture Collection is a nonprofit organization which collects, stores, and distributes standard reference microorganisms, cell lines and other materials for research and development. Established in 1925 to serve as a national center for depositing and distributing microbiological specimens, ATCC has since grown to ...
A549 may refer to: A549 (cell line), a carcinomic human alveolar basal epithelial cell; A549 road (Wales), a road in Great Britain This page was last edited on 27 ...