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Lambda phage is a non-contractile tailed phage, meaning during an infection event it cannot 'force' its DNA through a bacterial cell membrane. It must instead use an existing pathway to invade the host cell, having evolved the tip of its tail to interact with a specific pore to allow entry of its DNA to the hosts.
A cosmid is a type of hybrid plasmid that contains a Lambda phage cos sequence. [1] Often used as cloning vectors in genetic engineering, cosmids can be used to build genomic libraries. They were first described by Collins and Hohn in 1978. [2] Cosmids can contain 37 to 52 (normally 45) kb of DNA, limits based on the normal bacteriophage ...
[4] cII acts as a transcriptional activator of three promoters on the phage genome: pI, pRE, and pAQ. [3] cII is an unstable protein with a half-life as short as 1.5 mins at 37˚C, [5] enabling rapid fluctuations in its concentration. First isolated in 1982, [6] cII's function in lambda's regulatory network has been extensively studied.
Temperate phages (such as lambda phage) can reproduce using both the lytic and the lysogenic cycle. [4] How a phage decides which cycle to enter depends on a variety of factors. [5] For instance, if there are several other infecting phages (or if there is a high multiplicity), it is likely that the phage will use the lysogenic cycle.
The earliest identified members of the serine recombinase family were known as resolvases or DNA invertases, while the founding member of the tyrosine recombinases, lambda phage integrase (using attP/B recognition sites), differs from the now well-known enzymes such as Cre (from the P1 phage) and FLP (from the yeast Saccharomyces cerevisiae).
Lambdavirus (synonyms Lambda-like viruses, Lambda-like phages, Lambda phage group, Lambda phage) is a genus of viruses in the order Caudovirales, in the family Siphoviridae. Bacteria serve as natural hosts, with transmission achieved through passive diffusion. There are five species in this genus.
When the Integration Host Factor was first discovered, it was only known for the site-specific recombination of bacteriophage. [4] This is all we knew for a while but through another article, we were able to find that with further research, IHF plays a key role in the scope of physiological processes of E. Coli, including site-specific recombination activities, phage packaging and partitioning ...
Phages like the lambda phage use their site specific recombinases to integrate their DNA into the host genome during lysogeny. P1 phage DNA on the other hand, exists as a plasmid in the host. The Cre-lox system serves several functions in the phage: it circularizes the phage DNA into a plasmid, separates interlinked plasmid rings so they are ...