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  2. Nucleic acid thermodynamics - Wikipedia

    en.wikipedia.org/wiki/Nucleic_acid_thermodynamics

    For DNA oligonucleotides, i.e. short sequences of DNA, the thermodynamics of hybridization can be accurately described as a two-state process. In this approximation one neglects the possibility of intermediate partial binding states in the formation of a double strand state from two single stranded oligonucleotides.

  3. DNA - Wikipedia

    en.wikipedia.org/wiki/DNA

    The stability can be measured in various ways; a common way is the melting temperature (also called T m value), which is the temperature at which 50% of the double-strand molecules are converted to single-strand molecules; melting temperature is dependent on ionic strength and the concentration of DNA.

  4. Coding theory approaches to nucleic acid design - Wikipedia

    en.wikipedia.org/wiki/Coding_theory_approaches...

    DNA codes with constant GC-content can obviously be constructed from constant-composition codes (A constant composition code over a k-ary alphabet has the property that the numbers of occurrences of the k symbols within a codeword is the same for each codeword) over by mapping the symbols of to the symbols of the DNA alphabet, = {,,,}.

  5. DNA clamp - Wikipedia

    en.wikipedia.org/wiki/DNA_clamp

    The clamp-polymerase protein–protein interactions are stronger and more specific than the direct interactions between the polymerase and the template DNA strand; because one of the rate-limiting steps in the DNA synthesis reaction is the association of the polymerase with the DNA template, the presence of the sliding clamp dramatically ...

  6. Sticky and blunt ends - Wikipedia

    en.wikipedia.org/wiki/Sticky_and_blunt_ends

    DNA ends refer to the properties of the ends of linear DNA molecules, which in molecular biology are described as "sticky" or "blunt" based on the shape of the complementary strands at the terminus. In sticky ends , one strand is longer than the other (typically by at least a few nucleotides), such that the longer strand has bases which are ...

  7. DNA sequencing - Wikipedia

    en.wikipedia.org/wiki/DNA_sequencing

    Testing DNA is a technique which can detect specific genomes in a DNA strand to produce a unique and individualized pattern. DNA sequencing may be used along with DNA profiling methods for forensic identification and paternity testing , as it has evolved significantly over the past few decades to ultimately link a DNA print to what is under ...

  8. Eukaryotic DNA replication - Wikipedia

    en.wikipedia.org/wiki/Eukaryotic_DNA_replication

    Initiation of eukaryotic DNA replication is the first stage of DNA synthesis where the DNA double helix is unwound and an initial priming event by DNA polymerase α occurs on the leading strand. The priming event on the lagging strand establishes a replication fork.

  9. DNA sequencer - Wikipedia

    en.wikipedia.org/wiki/DNA_sequencer

    A DNA sequencer is a scientific instrument used to automate the DNA sequencing process. Given a sample of DNA, a DNA sequencer is used to determine the order of the four bases: G , C , A and T . This is then reported as a text string, called a read.