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  2. Staining - Wikipedia

    en.wikipedia.org/wiki/Staining

    A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue .

  3. Giemsa stain - Wikipedia

    en.wikipedia.org/wiki/Giemsa_stain

    Giemsa's solution is a mixture of methylene blue, eosin, and Azure B.The stain is usually prepared from commercially available Giemsa powder. A thin film of the specimen on a microscope slide is fixed in pure methanol for 30 seconds, by immersing it or by putting a few drops of methanol on the slide.

  4. Gram stain - Wikipedia

    en.wikipedia.org/wiki/Gram_stain

    A Gram stain of mixed Staphylococcus aureus (S. aureus ATCC 25923, gram-positive cocci, in purple) and Escherichia coli (E. coli ATCC 11775, gram-negative bacilli, in red), the most common Gram stain reference bacteria. Gram stain (Gram staining or Gram's method), is a method of staining used to classify bacterial species into two large groups ...

  5. Alcian blue stain - Wikipedia

    en.wikipedia.org/wiki/Alcian_blue_stain

    Micromass cultures of C3H-10T1/2 cells at varied oxygen tensions stained with Alcian blue. Alcian blue (/ ˈ æ l ʃ ə n /) is any member of a family of polyvalent basic dyes, of which the Alcian blue 8G (also called Ingrain blue 1, and C.I. 74240, formerly called Alcian blue 8GX from the name of a batch of an ICI product) has been historically the most common and the most reliable member. [1]

  6. Basophilic - Wikipedia

    en.wikipedia.org/wiki/Basophilic

    Main staining types when using hematoxylin and eosin (H&E). A Basophil granulocyte stains dark purple upon H&E staining. Basophilic is a technical term used by pathologists. It describes the appearance of cells, tissues and cellular structures as seen through the microscope after a histological section has been stained with a basic dye.

  7. Romanowsky stain - Wikipedia

    en.wikipedia.org/wiki/Romanowsky_stain

    Blood film stained with Giemsa showing Plasmodium (center of image), the parasite that causes malaria infections.. In 1891 Romanowsky [8] [9] [10] developed a stain using a mixture of eosin (typically eosin Y) and aged solutions of methylene blue that formed hues unattributable to the staining components alone: distinctive shades of purple in the chromatin of the cell nucleus and within ...

  8. Bismarck brown Y - Wikipedia

    en.wikipedia.org/wiki/Bismarck_brown_Y

    Bismarck brown Y also called C.I. 21000 [1] and C.I. Basic Brown 1, [1] is a diazo dye with the idealized formula [(H 2 N) 2 C 6 H 3 N 2] 2 C 6 H 4. The dye is a mixture of closely related compounds. It was one of the earliest azo dyes, being described in 1863 by German chemist Carl Alexander von Martius. It is used in histology for staining ...

  9. Periodic acid–Schiff stain - Wikipedia

    en.wikipedia.org/wiki/Periodic_acid–Schiff_stain

    PAS staining is also used for staining cellulose. One example would be looking for implanted medical devices composed of nonoxidized cellulose. If the PAS stain will be performed on tissue, the recommended fixative is 10% neutral-buffered formalin or Bouin solution. For blood smears, the recommended fixative is methanol.