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A UV-Vis spectrophotometer is an analytical instrument that measures the amount of ultraviolet (UV) and visible light that is absorbed by a sample. It is a widely used technique in chemistry, biochemistry, and other fields, to identify and quantify compounds in a variety of samples.
The Cary 14 spectrophotometer was first produced in 1954 by the Applied Physics Corporation, which later was named the Cary Instruments Corporation after co-founder Howard Cary. [1] The instrument was a successor to the Cary 11, which was the first commercially available recording UV/Vis spectrophotometer. [3]
There are some common types of spectrophotometers include: UV-vis spectrophotometer: Measures light absorption in UV and visible ranges (200-800 nm). Used for quantification of many inorganic and organic compounds. 1. Infrared spectrophotometer: Measures infrared light absorption, allowing identification of chemical bonds and functional groups. 2.
[17]: 18 Its designation as a "UV–Vis" spectrophotometer indicates its ability to measure light in both the visible and ultraviolet spectra. [ 29 ] The DU was the first commercially viable scientific instrument for measuring the amount of ultraviolet light absorbed by a substance.
A d 1 octahedral metal complex, such as [Ti(H 2 O) 6] 3+, shows a single absorption band in a UV-vis experiment. [7] The term symbol for d 1 is 2 D, which splits into the 2 T 2g and 2 E g states. The t 2g orbital set holds the single electron and has a 2 T 2g state energy of -4Dq.
If the instrument is designed to measure the spectrum on an absolute scale rather than a relative one, then it is typically called a spectrophotometer. The majority of spectrophotometers are used in spectral regions near the visible spectrum. A spectrometer that is calibrated for measurement of the incident optical power is called a ...
1 mL and 3 mL cuvettes. Traditional ultraviolet–visible spectroscopy or fluorescence spectroscopy uses samples that are liquid. Often the sample is a solution, with the substance of interest dissolved within. The sample is placed in a cuvette and the cuvette is placed in a spectrophotometer for testing.
UV–vis spectroscopy sees only chromophores, so other molecules must be prepared for analysis by chemical addition of a chromophore such as anthracene. Two methods are reported: the octant rule and the exciton chirality method. [1] The octant rule was introduced in 1961 by William Moffitt, R. B. Woodward, A. Moscowitz, William Klyne and Carl ...