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Electrons change energy states by either resonantly gaining energy from absorption of a photon or losing energy by emitting photons. In chemistry-related disciplines, one often distinguishes between fluorescence and phosphorescence. The former is typically a fast process, yet some amount of the original energy is dissipated so that re-emitted ...
The fluorescence of certain rocks and other substances had been observed for hundreds of years before its nature was understood. One of the first to explain it was Irish scientist Sir George Stokes from the University of Cambridge in 1852, who named the phenomenon "fluorescence" after fluorite, a mineral many of whose samples glow strongly because of impurities.
Many of the detection modes (absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization) are available stand-alone in dedicated plate readers, but are very often found today combined into one instrument (multi-mode plate reader).
Phosphorescent materials continue to emit light for some time after the radiation stops. This difference in timing is a result of quantum spin effects. Fluorescence occurs when a photon of the incoming radiation is absorbed by a molecule exciting it to a higher energy level followed by emission of light as the molecule returns to a lower energy ...
Luminescence is a spontaneous emission of radiation from an electronically or vibrationally excited species not in thermal equilibrium with its environment. [ 1 ] A luminescent object emits cold light in contrast to incandescence , where an object only emits light after heating. [ 2 ]
Phosphorescence is a type of photoluminescence related to fluorescence. When exposed to light (radiation) of a shorter wavelength, a phosphorescent substance will glow, absorbing the light and reemitting it at a longer wavelength. Unlike fluorescence, a phosphorescent material does not immediately reemit the radiation it absorbs.
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