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The 50% cutoff frequency is determined to yield the corresponding spatial frequency. Thus, the approximate position of best focus of the unit under test is determined from this data. The MTF data versus spatial frequency is normalized by fitting a sixth order polynomial to it, making a smooth curve.
As an example, a telescope having an f /6 objective and imaging at 0.55 micrometers has a spatial cutoff frequency of 303 cycles/millimeter. High-resolution black-and-white film is capable of resolving details on the film as small as 3 micrometers or smaller, thus its cutoff frequency is about 150 cycles/millimeter.
In electronics, cutoff frequency or corner frequency is the frequency either above or below which the power output of a circuit, such as a line, amplifier, or electronic filter has fallen to a given proportion of the power in the passband.
Such a probe has a high signal collection efficiency (>90%) and no frequency cutoff. [21] Another alternative is "active tip" schemes, where the tip is functionalized with active light sources such as a fluorescent dye [22] or even a light emitting diode that enables fluorescence excitation. [23]
The effect of the contrast transfer function can be seen in the alternating light and dark rings (Thon rings), which show the relation between contrast and spatial frequency. The contrast transfer function (CTF) mathematically describes how aberrations in a transmission electron microscope (TEM) modify the image of a sample.
The half-power point is the point at which the output power has dropped to half of its peak value; that is, at a level of approximately −3 dB. [1] [a]In filters, optical filters, and electronic amplifiers, [2] the half-power point is also known as half-power bandwidth and is a commonly used definition for the cutoff frequency.
Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
The stopband of a low-pass filter is the frequencies from the stopband corner frequency (which is slightly higher than the passband 3 dB cut-off frequency) up to the infinite frequency. The stopband of a high-pass filter consists of the frequencies from 0 hertz to a stopband corner frequency (slightly lower than the passband cut-off frequency).
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