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In molecular biology, enzymes in the DNA/RNA non-specific endonuclease family of bacterial and eukaryotic endonucleases EC 3.1.30.-share the following characteristics: they act on both DNA and RNA, cleave double-stranded and single-stranded nucleic acids and require a divalent ion such as magnesium for their activity.
The rate of ERCC1 protein turnover also does not correlate with ERCC1 protein level. A translational level control of ERCC1, due to a microRNA (miRNA), has been shown during HIV viral infection. A trans-activation response element (TAR) miRNA, coded for by the HIV virus, down-regulates ERCC1 protein expression. [43] TAR miRNA allows ERCC1 mRNA ...
The non-specific DNA cleavage domain from the end of the FokI endonuclease can be used to construct hybrid nucleases that are active in a yeast assay. [6] [7] These reagents are also active in plant cells [8] [9] and in animal cells.
Evidence suggests that endonuclease activity experiences a lag compared to exonuclease activity. [2] Restriction enzymes are endonucleases from eubacteria and archaea that recognize a specific DNA sequence. [3] The nucleotide sequence recognized for cleavage by a restriction enzyme is called the restriction site.
It was cloned in 1987 and shown to consist of a 266 protein precursor, [6] which is further cleaved and secreted as a 245 amino acid active nuclease. [7] Its active form in solution is a homodimer. [8] It has two disulfide bonds, the first between cysteine 30 & 34 and the second between cysteine 222 & 264. [7]
ERCC4 is a protein designated as DNA repair endonuclease XPF that in humans is encoded by the ERCC4 gene. Together with ERCC1, ERCC4 forms the ERCC1-XPF enzyme complex that participates in DNA repair and DNA recombination. [5] [6] The nuclease enzyme ERCC1-XPF cuts specific structures of DNA. Many aspects of these two gene products are ...
13804 Ensembl ENSG00000167136 ENSMUSG00000015337 UniProt Q14249 O08600 RefSeq (mRNA) NM_004435 NM_007931 RefSeq (protein) NP_004426 NP_031957 Location (UCSC) Chr 9: 128.82 – 128.82 Mb Chr 2: 30.06 – 30.06 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Endonuclease G, mitochondrial is an enzyme that in humans is encoded by the ENDOG gene. This protein primarily participates in ...
(Further reading: Homing endonuclease § Structural families.) PDB code: Code used to identify the structure of a protein in the PDB database. If no structure is available, a UniProt identifier is given instead. Source: Organism that naturally produces the enzyme. D: Biological domain of the source: A: archaea – B: bacteria – E: eukarya.