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Bis-tris propane, or 1,3-bis(tris(hydroxymethyl)methylamino)propane, also known as BTP, is a chemical substance that is used in buffer solutions. It is a white to off-white crystalline powder that is soluble in water. It has a wide buffering range, from 6 to 9.5 due to its two pK a values which are close in value.
Bis-tris methane, also known as BIS-TRIS or BTM, is a buffering agent used in biochemistry. Bis-tris methane is an organic tertiary amine with labile protons having a pKa of 6.46 at 25 °C. It is an effective buffer between the pH 5.8 and 7.2. Bis-tris methane binds strongly to Cu and Pb ions as well as, weakly, to Mg, Ca, Mn, Co, Ni, Zn and Cd ...
The buffer should also be unreactive and not modify or react with most proteins. Different buffers may be used as cathode and anode buffers, respectively, depending on the application. Multiple pH values may be used within a single gel, for example in DISC electrophoresis. Common buffers in PAGE include Tris, Bis-Tris, or imidazole.
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Top Chef winner and host Kristen Kish is joining the TODAY Food team to kick off the 21st season of the hit cooking competition show. To celebrate joining as a judge and the start of the new ...
Indirect grilling is designed to cook larger (e.g. pork shoulders, whole chicken) or tougher foods (e.g. brisket, ribs) that would burn if cooked using a direct flame. This method of cooking generates a more moderate temperature (about 275–350 °F or 135–177 °C) and allows for an easier introduction of wood smoke for flavoring.
The useful buffer range for tris (pH 7–9) coincides with the physiological pH typical of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory. Tris is also used as a primary standard to standardize acid solutions for chemical analysis.
TAPS ([tris(hydroxymethyl)methylamino]propanesulfonic acid) is a chemical compound commonly used to make buffer solutions. It can bind divalent cations, including Co(II) and Ni(II). [1] TAPS is effective to make buffer solutions in the pH range 7.7–9.1, since it has a pK a value of 8.44 (ionic strength I = 0, 25 °C). [2]